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High Efficiency Cloning and Expression System for Proteomic Analysis

机译：蛋白质组学分析的高效克隆和表达系统

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The recent description of the complete genomes of the two most pathogenic species of Brucella opens the way for genome-based analysis of the antigenicity of their proteins. In the present report, we describe a bench- level high-efficiency cloning and expression system (HECES) that allow expression of large numbers of Brucella proteins based on genomic sequence information. Purified proteins are produced with high efficiency in a microarray format conducive to analysis of their sero-reactivity against serum from immunized animals. This method is applicable at either small or large scale of protein processing. while it does not require robotics, the format is amenable to robotic implementation for all aspects of the process and subsequent analysis of protein characteristics. This method will allow selection of new reagents for diagnosis of brucellosis and development of vaccine against Brucella, an important zoonotic disease and biothreat agent.

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Ding, X. Z. ; Paulsen, I. T. ; Bhattacharjee, A. K. ; Nikolich, M. P. ; Myers, G.;
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年度 2006
页码 1-10
总页数 10
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Clones; Antigens; Brucella; Brucellosis; Pathogenic microorganisms; Genome; Robotics; High rate; Processing; Proteins; Vaccines; Zoonotic diseases; Genetic mapping; Animals; Genetic engineering; Efficiency; Diagnosis(Medicine); Purification; Chemical agents;

机译：克隆;抗原;布鲁氏菌;布鲁氏菌病;病原微生物;基因组;机器人;高比率;加工;蛋白质;疫苗;人畜共患病;遗传作图;动物;基因工程;效率;诊断（医学）;纯化;化学药剂;

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1. A high efficiency cloning and expression system for proteomic analysis [J] . Ding XZ, Paulsen IT, Bhattacharjee AK, Proteomics . 2006,第14期

机译：用于蛋白质组学分析的高效克隆和表达系统
2. High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system [J] . Blommel PG, Martin PA, Wrobel RL, Protein Expression and Purification . 2006,第2期

机译：使用Flexi Vector克隆系统从cDNA克隆高效一步生产表达质粒
3. Molecular Cloning and Expression of a cDNA Encoding a Coccidioides posadasii Cu,Zn Superoxide Dismutase Identified by Proteomic Analysis of the Coccidioidal T27K Vaccine [J] . JENNINE M. LUNETTA, KEIRA A. SIMMONS, SUZANNE M. JOHNSON, Annals of the New York Academy of Sciences . 2007,第Sep期

机译：通过球蛋白T27K疫苗的蛋白质组学分析鉴定了编码球孢子虫铜，锌超氧化物歧化酶的cDNA的分子克隆和表达。
4. Differential Protein Expression Induced by c-Myc Over-Expression: Proteomic Analysis of a CHO Cell Line with Increased Proliferation Capacity [C] . Darrin Kuystermans and Mohamed Al-Rubeai European Society of Animal Cell Technology . 2010

机译：C-Myc过表达诱导的差异蛋白质表达：CHO细胞系蛋白质组学分析，增殖能力增加
5. Studies of schistosome DNA: Species and strain differences from melting and drug resistance DNA analysis and cloning, sequencing and expression analysis of a putative praziquantel binding Ca(++)-ATPase gene. [D] . Tsai, Meng-Hsiun. 1998

机译：血吸虫DNA的研究：物种和菌株差异从融化和耐药性DNA分析以及推定的吡喹酮结合Ca（++）-ATPase基因的克隆，测序和表达分析。
6. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. [O] . A Aruffo, B Seed 1987

机译：通过高效的COS细胞表达系统分子克隆CD28 cDNA。
7. A high efficiency cloning and expression system for proteomic analysis [O] . Ding Xuan, Paulsen Ian, Bhattacharjee Apurba, 2006

机译：用于蛋白质组学分析的高效克隆和表达系统

High Efficiency Cloning and Expression System for Proteomic Analysis

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