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Structure of the Chromophore within a Red Fluorescent Protein from Zoanthus sp

机译：来自Zoanthus sp。的红色荧光蛋白中发色团的结构

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This report results from a contract tasking Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS as follows: During the past decade, Green Fluorescent Protein (GFP) has become one of the most widely used fluorescent probes that enable direct visualization of the intracellular processes in the living cell. The discovery of the red-emitting GFP-like proteins from Anthozoa species promises considerable broadening of the scope of biochemical applications of these probes. Further rational designing of new red- emitting probes with desired optical properties is tightly associated with understanding of the chemical background of the red shift phenomenon. Recently, three highly homologous green (zFP506), yellow (zFP538) and red (zFP576) fluorescent proteins from Zoanthus sp. were cloned. More recently it was shown, that in contrast to GFP-like chromophore structure of zFP506, the chromophore of the yellow fluorescent protein zFP538 undergoes additional modification of a transiently appearing DsRed-like structure. Our preliminary studies indicate that the red fluorescent protein from Zoanthus sp. zFP576 apparently contains the chromophore differing from both zFP538 and DsRed. The proposed research is aimed at the study of zFP576 chromophore structure.

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