首页> 美国政府科技报告 >Smart Gold Nanobowls (Nano-Crescent Moon) with Sub-10 nm Circular Edge for Local Electromagnetic Field Enhancement Effect, Spatial, and NIR Temporal/Thermal Modulations for Molecular and Cellular Dynamic Imaging
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Smart Gold Nanobowls (Nano-Crescent Moon) with Sub-10 nm Circular Edge for Local Electromagnetic Field Enhancement Effect, Spatial, and NIR Temporal/Thermal Modulations for Molecular and Cellular Dynamic Imaging

机译:具有亚10nm圆形边缘的智能金纳米球(纳米月牙),用于局部电磁场增强效果,空间和NIR时间/热调制,用于分子和细胞动态成像

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Cell signaling involves the production and activation of many intracellular biomolecules. The dynamic detection of the biomolecular reactions is essential to understand the undefined signaling pathway. Current fluorescence based methods have difficulty detecting the dynamic reactions of biomolecules such as nucleolytic, proteolytic, and phosphorylation. In contrast, label-free Raman scattering spectroscopy can detect subtle biochemical changes without fluorescent molecules. First, we have created gold-based nanocrescent Surface Enhanced Raman Scattering (SERS) probes which can function as the substrate to enhance Raman scattering signal for tens of orders of magnitude. Once functionalized with oligonucleotides and peptides, the internalized nanocrescent particles can be used to monitor the enzymes inside the single living cell. In the past ten months, in addition to gold-based nanocrescent SERS probes, we have accomplished the multilayer composite nanocrescent SERS probes with magnetic thin film layer inside of gold nanocresents.

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