Expression and Genomic Profiling of Minute Breast Cancer Samples

机译：微小乳腺癌样本的表达和基因组分析

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To be able to apply the newest genetic analysis technologies in breast cancer research, enough DNA material must be available to perform this analysis. Often this is impossible with the minute amounts of tissue obtained via fine needle aspiration or laser capture microdissection currently the best methods available for removal of small amounts of tissue. Therefore whole genome-based DNA amplification techniques (e.g. PCR) are essential. The aim of this proposal was to evaluate a newly developed method, balanced PCR, which overcomes the difficulty of non-linear PCR-amplification of complex genomes and faithfully retains the difference among corresponding genes or gene fragments. In the three years of work we demonstrated the application of balanced PCR in performing genomic profiling of breast CA cell lines and samples (part of Tasks 2 and 3). In the second year we demonstrated the application of the method for gene expression profiling of breast CA cell lines (Task 1). This report describes the work conducted over the three years.

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作者
Makrigiorgos, G. M.;
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年度 2006
页码 1-70
总页数 70
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Genetics; Breast cancer; Genome; Materials; Lasers; Deoxyribonucleic acids; Sampling; Fragments; Mammary glands; Genetic tissue identification; Needles; Aspirators; Genetic mapping; Genes; Amplification; Medical research; Cells(Biology);

机译：遗传学;乳腺癌;基因组;材料;激光;脱氧核糖核酸;取样;片段;乳腺;遗传组织鉴定;针;吸气器;遗传作图;基因;扩增;医学研究;细胞（生物学）;

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2. Inter-tumor genomic heterogeneity of breast cancers: comprehensive genomic profile of primary early breast cancers and relapses [J] . Caterina Fumagalli, Alberto Ranghiero, Sara Gandini, Breast Cancer Research . 2020,第1期

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机译：双乳腺癌和脑转移之间的不同基因表达谱与双匹配样品
4. Comparative Study on Data Mining Techniques Applied to Breast Cancer Gene Expression Profiles [C] . Sergio Mosquim Junior., Juliana de Oliveira International Conference on Bioinformatics Models, Methods and Algorithms . 2017

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5. Comparing gene expression profiles of cell culture models with profiles of tumor xenografts and patient tumors: Implications for lung and breast cancers. [D] . Creighton, Chad J. 2006

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6. Distinct gene expression profiles between primary breast cancers and brain metastases from pair-matched samples [O] . Takayuki Iwamoto, Naoki Niikura, Rin Ogiya, -1

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7. Distinct gene expression profiles between primary breast cancers and brain metastases from pair-matched samples [O] . Takayuki Iwamoto, Naoki Niikura, Rin Ogiya, 2019

机译：双乳腺癌和脑转移之间的不同基因表达谱与双匹配样品

Expression and Genomic Profiling of Minute Breast Cancer Samples

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