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首页> 美国政府科技报告 >Global Alterations in Gene Expression During Organophosphate Pesticide Intoxication and Recovery: Interim Report
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Global Alterations in Gene Expression During Organophosphate Pesticide Intoxication and Recovery: Interim Report

机译：有机磷农药中毒和恢复期间基因表达的全球变化：中期报告

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The effects of low-level, transient exposures to organophosphate pesticides (OPP) and chemically related nerve agents (e.g., sarin, soman, tabun) remain difficult to detect. Because Caenorhabditis elega is relatively resistant to OPP lethality, studies in this nematode provide an opportunity to observe alterations in global gene expression following OPP exposure that cannot be readily observed in less resistant organisms. It may be possible to highlight changes in gene expression that might be important in low-level, transient exposures. Conventional culture techniques for C. elegans use bacteria as food source. The presence of the bacteria may confound interpreting the effects of a test substance if the substance is metabolized by the bacteria. Further, it can be difficult to purify worm nucleic acids away from those of the bacteria for analysis. We developed a liquid medium, CeHR medium, to facilitate the propagation of worms in the absence of bacteria. We exposed cultures of worms in CeHR medium to 2 doses of dichlorvos under 3 exposure protocols. In the first, worms were exposed continuously throughout the experiment. In the second and third, worms were exposed for 2 or 8 h, the dichlorvos was washed out of the culture, and the worms were allowed to recover. We then harvested RNA for global gene expression studies.

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