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首页> 美国政府科技报告 >Analysis of Breast Cell-Lineage Response Differences to Taxol Using a Novel Co-Culture System
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Analysis of Breast Cell-Lineage Response Differences to Taxol Using a Novel Co-Culture System

机译：利用新型共培养体系分析乳腺细胞谱系对紫杉醇的反应差异

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We have established a new co-culture system in which human mammary epithelial cells (HMEC) and human mammary tumor cells (HMT) are physically grown together. We hypothesized that cells in co-culture (CC) would generate gene expression profiles different from homogeneous cell populations. In this study, cells were incubated with blue or red CellTracker Dyes and co-cultured. Our novel capture system allowed cells to be co-cultured and then quickly separated while maintaining 90% viability. Using deconvolution microscopy, co- cultured HMEC were observed to form focal, gland-like structures surrounded by TTUderived from an invasive ductal carcinoma. Using a differential trypsinization technique, cell populations were rapidly separated to perform RNA extractions performed.

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