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首页> 美国政府科技报告 >Hemorrhagic Shock-Induced Vascular Hyporeactivity in the Rat: Relationship to Gene Expression of Nitric Oxide Synthase, Endothelin-1, and Select Cytokines in Corresponding Organs.
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Hemorrhagic Shock-Induced Vascular Hyporeactivity in the Rat: Relationship to Gene Expression of Nitric Oxide Synthase, Endothelin-1, and Select Cytokines in Corresponding Organs.

机译：出血性休克诱导的大鼠血管低反应性：与相应器官中一氧化氮合酶，内皮素-1和选择细胞因子的基因表达的关系。

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Background. Our previous work observed that vascular hyporeactivity to norepinephrine (NE) developed after hemorrhage and the response was not the same in the 4 arteries examined. To evaluate possible mechanisms involved, the present study investigated the gene expression of iNOS, eNOS, IL-1 Beta , IL-6, TNF-alpha , and endothelin-1 in the corresponding organs, and the roles of nitric oxide (NO) and endothelin (ET). Materials and methods. LAnesthetized rats (n = 7/time point/group) were hemorrhaged to a mean arterial pressure of 50 mmHg for 60 min. The vascular reactivity of the superior mesenteric (SMA), celiac (CA), left renal (LRA), and left femoral arteries (LFA) to NE was measured at baseline, at the end of the hypotensive period (E), and at 1, 2, an d 4 h later in the three groups (hemorrhage, hemorrhage NG-nitro-L-arginine methyl ester (L -NAME), an NO synthase inhibitor, or hemorrhage PD142893, an ET receptor antagonist). Gene expression inileum, left kidney, liver, and skeletal muscle was determined by quantitative RT-PCR at these times. Results. Vascular reactivity of SMA, CA, LRA, and LFA to NE decreased as much as 98% over 4 h compared with baseline. This loss of responsiveness in CA and LFA was more severe than in SMA and LRA. Gene expression of iNOS, eNOS, IL-1 beta, IL-6, TNF-alpha, and endothelin-1 in the corresponding organs of select vasculatures increased markedly over baseline levels and the fold increase in mRNA levels of these enzymes and mediators in liver and skeletal muscle was higher than in ileum and left kidney. For example, at 4 h, iNOS expression was over 16-fold higher than baseline in liver and skeletal muscle, but 5- and 7-fold higher in ileum and kidney, respectively. L -NAME or PD142893 partially attenuated the decreased vascular reactivity induced by hemorrhagic shock and attenuated the changes in gene expression observed.

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