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DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis.

机译：DNase 1在金纳米簇合成后保留内切脱氧核糖核酸酶活性。

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Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 microg/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.

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West, A. L.; Griep, M. H.; Cole, D. P.; Karna, S. P.;
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年度 2014
页码 1-7
总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Fluorescence; Gold; Ribonuclease; Assaying; Biomolecules; Enzyme chemistry; Synthesis(Chemistry); Gold nanoclusters; Fluorescence emissions; Dna contamination detection; Plasmonic transitions; Endodeoxyribonuclease activity;

机译：荧光;金;核糖核酸酶;测定;生物分子;酶化学;合成（化学）;金纳米簇;荧光发射; Dna污染检测;等离子体跃迁;内切脱氧核糖核酸酶活性;

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专利

1. DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis [J] . Abby L. West, Mark H. Griep, Daniel P. Cole, Analytical chemistry . 2014,第15期

机译：DNA金纳米簇合成后，DNase 1保留了内脱氧核糖核酸酶活性。
2. Polydopamine Nanosphere/Gold Nanocluster (Au NC)-Based Nanoplatform for Dual Color Simultaneous Detection of Multiple Tumor-Related MicroRNAs with DNase-I-Assisted Target Recycling Amplification [J] . Xu Shenghao, Nie Yongyin, Jiang Liping, Analytical chemistry . 2018,第6期

机译：聚二胺纳米球纳米纳米纳米粉（Au NC）基础纳米片，用于双色同时检测多种肿瘤相关的微大RNA，DNase-I辅助的靶再循环扩增
3. Gold Nanocluster DNase 1 Hybrid Materials: An Efficient Method for DNA Contamination Sensing. [J] . West Abby L., Griep Mark H., Cole Dan P., Nanotechnology Magazine, IEEE . 2015,第1期

机译：金纳米簇DNase 1杂化材料：DNA污染传感的有效方法。
4. Gold nanocluster-DNase 1 hybrid materials for DNA contamination sensing [C] . West A.L., Griep M.H., Cole D.P., IEEE International Conference on Nanotechnology . 2014

机译：金纳米簇-DNase 1杂合材料，用于DNA污染检测
5. Structure and reactivity studies of size-selected transition metal sulfide nanoclusters in the gas phase and supported on gold(111). [D] . Patterson, Melissa Jean. 2009

机译：负载在金上的尺寸选择的过渡金属硫化物纳米簇的结构和反应性研究（111）。
6. Protein-Protected Gold Nanocluster-Based Biosensorfor Determining the Prooxidant Activity of Natural Antioxidant Compounds [O] . Esin Akyüz, Furkan Burak Şen, Mustafa Bener, 2019

机译：基于蛋白质的金纳米簇生物传感器测定天然抗氧化剂化合物的抗氧化活性
7. Glycolytic function in goldfish hepatocytes at different temperatures: relevance for Na+ pump activity and protein synthesis. [O] . G Krumschnabel, S Malle, P J Schwarzbaum, 1994

机译：不同温度下金鱼肝细胞的糖酵解功能：Na +泵活性和蛋白质合成的相关性。

DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis.

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