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首页> 美国政府科技报告 >Proteomics Analysis of Molecular Mechanisms of Multidrug Resistance in Breast Cancer Chemotherapy
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Proteomics Analysis of Molecular Mechanisms of Multidrug Resistance in Breast Cancer Chemotherapy

机译：蛋白质组学分析乳腺癌化疗中多药耐药的分子机制

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Recently breast cancer resistance protein (BCRP) has been found to be a frequent cause of MDR by causing increased efflux of a wide variety of cytotoxic drugs. Although it has been shown that transfection of BCRP into breast cancer cell line MCF7 caused drug resistance, it has also been found that the drug resistance level of these cells were much lower than that of the drug-selected cells. Thus, there must be other drug resistant mechanisms in the drug selected MCF7/AdrVp cells. This study is designed to test this concept. Specifically, we plan to achieve the following objectives using proteomics technology: (a) to compare protein profiles between MCF7 and MCF7/AdrVp cells using two-dimensional gel analysis, (b) to identify the proteins of different levels between the two cell lines using MALDI-TOF mass spectrometry analysis, (c) to confirm the different level of the identified proteins using western blot, and (d) to test the role of these proteins in mediating MDR using MTT assay. The information and probes obtained from this study will help us understand the molecular mechanism of drug resistance in breast cancer cells. This work may also help us discover new therapeutics for treating drug resistant breast tumors.

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