• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国政府科技报告 >Estrogen Receptor-Selective Coactivator: A Potential Regulator of Tamoxifen Effectiveness in Breast Cancer Treatment and Prevention
【24h】

Estrogen Receptor-Selective Coactivator: A Potential Regulator of Tamoxifen Effectiveness in Breast Cancer Treatment and Prevention

机译：雌激素受体选择性辅激活因子：三苯氧胺对乳腺癌治疗和预防有效性的潜在调节剂

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The estrogen receptor (ER) is involved in breast cancer development and progression. We have isolated a novel 97 kDa DEAD box RNA helicase (DP97) that interacts with the estrogen receptor alpha (ERa) in the presence of either estradiol or trans-hydroxytamoxifen. DP97 represses the transcriptonal activity of the estradiol-occupied estrogen receptor and this repression can be relieved with Trichostatin A(TSA), a selective histone deacetylase inhibitor. DP97 represses the activity of a constitutive SV4O promoter when it is recruited as a Gal-4 DNA binding domain fusion protein. DP97 also interacts with, and represses the activity of, other nucelar receptors such as the progesterone receptor b, glucocorticoid receptor, and retionic acid receptor a. However, DP97 does not repress the activity of either p53 or VP16. We show that the N- terminal helicase region of DP97 is dispensible for its activity as a transcriptional repressor. Furthermore, a BLAST homology search of the remaining sequence of p97 reveales that this protein has a small region of homology with the SMRTe(NCoR2) corepressor protein. This small region is also able to repress a constitutively active SV4O promoter when it is recmited to it as a Gal-4 DNA binding domain fusion protein. Therefore, DP97 functions as a RNA helicase, a nuclear receptor interacting protein, and as a transcriptional coregulator.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号