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首页> 美国政府科技报告 >Construction and Characterization of Human Mammary Epithelial Cell Lines Containing Mutations in the p53 or BRCA1 Genes
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Construction and Characterization of Human Mammary Epithelial Cell Lines Containing Mutations in the p53 or BRCA1 Genes

机译：含有p53或BRCa1基因突变的人乳腺上皮细胞系的构建和表征

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The overall goal of this project is to identify and characterize the consequences of human mammary epithelial cells (HMEC) that become deficient in normal p53 or BRCA-l gene functions. We have created retroviral vectors which allow us conditionally express the E6/E7 gene of human papillomavirus type 16 (HPVl6), dominant-negative p53 gene, or anti-sense BRCA-l gene. The consequences of transduction of these viral constructs into primary human mammary epithelial cells will be discovered through controlled in vitro comparisons between genetically altered derivatives and their isogenic parent cells. As we proposed in our last report, we are now focused our efforts on microarray-based comparisons to identify breast cancer related genes. During the past year we have successfully implemented the Microarray Spotting and Scanning techniques. This includes development of robust fluorescent labeling and hybridization protocols as well as the preparation and testing of over 23,000 minimally redundant cDNA target samples for deposition on the microarray slides. We have compared expression profiles from several distinct breast cell lines we had created. We believe that this system will provide us critical information to our understanding of early breast carcinogenesis.

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