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首页> 美国政府科技报告 >Compositional Heterogeneity Within Oceanic POM: A Study Using Flow Cytometry and Mass Spectrometry
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Compositional Heterogeneity Within Oceanic POM: A Study Using Flow Cytometry and Mass Spectrometry

机译：海洋pOm中的成分异质性：使用流式细胞术和质谱法的研究

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This thesis applied direct temperature-resolved mass spectrometry (DT-MS), flow cytometry, and multivariate statistics to the study of marine particulate organic matter (POM) collected from the North Atlantic. DT-MS is a rapid and sensitive molecular-level analytical technique. The fact that DT-MS only requires microgram quantities of sample permits the coupling of DT-MS and preparative flow cytometry. Flow cytometry was used here to isolate 'phytoplankton' and 'detritus' in 2 to 53 microns POM. The molecular-level differences between and within small-particle POM (<53 microns), large-particle POM (>53 micrlns), 'phytoplankton' and 'detritus' were explored using DT-MS and discriminant analysis. As the polysaccharide composition of POM subclasses was a major source of variation, polysaccharides in selected samples were further studied using ammonia CI(+) DT-MS. Principal component analysis of the resulting NH3-CI(+) spectra indicated that the majority of polysaccharide variation in the selected samples could be explained by a component related to the degree of degradation of the organic matter. The results from this thesis, coupled with existing work on marine organic matter, were used to support a modified 'size- reactivity continuum model' of organic matter cycling.

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