首页> 美国政府科技报告 >Rejuvenation and Freezing of Additive-Preserved Red Blood Cells in the 1000 ML-600 ML Polyvinyl Chloride Freezing Bay System Stored for Up to 42 Days at 4 C Prior to Rejuvenation and Glycerolization Using 40% W/V Glycerol and Storage at -80 C, Washed in the Haemonetics Blood Processor 115, and Stored at 4 C for up to 24 Hours Prior to Transfusion
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Rejuvenation and Freezing of Additive-Preserved Red Blood Cells in the 1000 ML-600 ML Polyvinyl Chloride Freezing Bay System Stored for Up to 42 Days at 4 C Prior to Rejuvenation and Glycerolization Using 40% W/V Glycerol and Storage at -80 C, Washed in the Haemonetics Blood Processor 115, and Stored at 4 C for up to 24 Hours Prior to Transfusion

机译:1000-mL-600 mL聚氯乙烯冷冻湾系统中添加保存的红细胞的复原和冷冻,在恢复和甘油化之前,在4℃下储存长达42天,使用40%W / V甘油并在-80℃储存,在Haemonetics血液处理器115中洗涤,并在输血前在4℃储存长达24小时

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摘要

This is a Standard Operating Procedure (Sop) to rejuvenate, glycerolize and deglycerolize human red cells collected into anticoagulant preservative (additive) systems. Current red blood cell additive systems include CPD-ADSOL (Fenwal Co.), CPD-Optisol (Terumo), and CP2D- Nutricel (MedSep) . Red blood cells collected into these systems can be stored at 4 deg C for up to 42 days prior to rejuvenation and freezing in the 1000-600 ml polyvinyl chloride (PVC) plastic bag. The rejuvenated, glycerolized red cells are concentrated and the supernatant glycerol removed prior to freezing. The rejuvenated, glycerolized red cells are washed in the Haemonetics Blood Processor 115.

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