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Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes

机译：用寡核苷酸探针测量细胞特异性核糖体RNa含量的细菌活性

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We describe a procedure for measuring the cell-specific quantity of ribosomal RNA (rRNA) and DNA in order to evaluate the frequency distribution of activity among cells. The procedure is inherently quantitative, does not require sample incubation and potentially can be taxon-specific. Fluorescently-labelled oligonucleotide probes are hybridized to the complementary 16S rRNA sequences in preserved, intact cells. The resulting cell fluorescence is proportional to cellular rRNA content and can be measured with a microscope-mounted photometer system, by image analysis, or by flow cytometry. Similarly, DNA content is measured as fluorescence of cells stained with the DNA specific fluorochrome DAPI. These are either prepared as separate samples for purposes of enumeration and DNA measurements, or are dual-labelled cells which are also hybridized with oligonucleotide probes.

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Kemp, P F; Lee, S; LaRoche, J;
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年度 1992
页码 1-18
总页数 18
原文格式 PDF
正文语种 eng
中图分类工业技术;
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Oligonucleotide probes; EDB/550200; EDB/550700;

机译：寡核苷酸探针; EDB / 550200; EDB / 550700;

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1. Detection of a single bacterial cell using a 16S ribosomal RNA-specific oligonucleotide probe designed to investigate periodontal pathogens. [J] . Tsuruda K, Shimazu A, Sugai M Oral microbiology and immunology . 2009,第2期

机译：使用旨在研究牙周病原体的16S核糖体RNA特异性寡核苷酸探针检测单个细菌细胞。
2. Evaluation of sequence alignments and oligonucleotide probes with respect to three-dimensional structure of ribosomal RNA using ARB software package [J] . Yadhu Kumar, Ralf Westram, Peter Kipfer, BMC Bioinformatics . 2006,第1期

机译：使用ARB软件包对核糖体RNA三维结构的序列比对和寡核苷酸探针的评价
3. Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin-embedded tissue sections and their rapid identification in bacterial smears. [J] . S Nordentoft, H Christensen, H C Wegener Journal of Clinical Microbiology . 1997,第10期

机译：评价针对23S rRNA的荧光标记寡核苷酸探针在石蜡包埋的组织切片中原位检测沙门氏菌血清及其在细菌涂片中的快速鉴定。
4. A MODEL FOR MEASURING MICROBIAL GROWTH IN ACTIVATED SLUDGE USING OLIGONUCLEOTIDE HYBRIDIZATION PROBES TARGETING 16S RIBOSOMAL RNA AND PRECURSOR 16S RIBOSOMAL RNA [C] . DANIEL B. OERTHER, LUTGARDE RASKIN Water Environment Federation 72nd annual conference amp; exposition (WEFTEC'99) . 1999

机译：以16S核糖体RNA和前体16S核糖体RNA为靶点的寡核苷酸杂交探针测定活性污泥中微生物生长的模型。
5. Analysis of the population structure of Acanthamoeba and the development of diagnostic oligonucleotide probes based upon nuclear small subunit ribosomal RNA gene sequences [D] . Gast, Rebecca Jane 1994

机译：棘阿米巴的种群结构分析和基于核小亚基核糖体RNA基因序列的诊断性寡核苷酸探针的开发
6. Variations of Bacterial Populations in Human Feces Measured by Fluorescent In Situ Hybridization with Group-Specific 16S rRNA-Targeted Oligonucleotide Probes [O] . Alison H. Franks, Hermie J. M. Harmsen, Gerwin C. Raangs, 1998

机译：组特异性16S rRNA靶向的寡核苷酸探针通过荧光原位杂交测量人类粪便中细菌种群的变化。
7. Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes [O] . P.F. Kemp, S. Lee, J. LaRoche 1992

机译：评估用寡核苷酸探针测量的细胞特异性核糖体RNA含量的细菌活性
8. Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes [R] . Kemp, P. F., Lee, S., LaRoche, J. 1992

机译：用寡核苷酸探针测量细胞特异性核糖体RNa含量的细菌活性

Evaluating bacterial activity from cell-specific ribosomal RNA content measured with oligonucleotide probes

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