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Preparative-Scale Proteins Seperations by Continuous Annular Chromatography.

机译:连续环形色谱法制备标准蛋白质分离度。

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The use of continuous annular chromatography (CAC) for the separation of protein mixtures is studied in a preparative-scale CAC unit. S-Sepharose, a strong-acid porous cation exchange resin is used as the separation medium mixtures of albumin, hemoglobin, and cytochrome-C are used as a model separation system. Equilibrium and mass transfer parameter are developed for this system on the basis of fixed-bed chromatograph experiments. A mathematical model is then successfully used in conjunction with these parameters to simulate the performance of the CAC separations. 11 refs., 11 figs., 3 tabs.

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