首页> 美国政府科技报告 >Study of Embryogenesis of Tsetse Flies after Mating with Irradiated Males. Part of a Coordinated Programme on Tsetse Fly Control or Eradication by the Sterile Male Technique. Final Report for the Period 15 December 1978-14 March 1
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Study of Embryogenesis of Tsetse Flies after Mating with Irradiated Males. Part of a Coordinated Programme on Tsetse Fly Control or Eradication by the Sterile Male Technique. Final Report for the Period 15 December 1978-14 March 1

机译:辐照雄性交配后采采蝇胚胎发生的研究。通过无菌雄性技术进行采采蝇控制或根除的协调计划的一部分。 1978年12月15日至14月1日期间的最终报告

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Seven to ten-day old males of G. p. palpalis (Nigerian origin) were irradiated in air with 12-13 Krad gamma rays from a exp 60 Co source. Three hours after treatment they were allowed to mate with two-day old normal females. Reproductive organs from females mated with treated males and with normal males were removed at regular intervals (starting on the 8th day following emergence) and examined by histological techniques. Extruded eggs were also examined. Development of most eggs fertilized by sperm of irradiated males was inhibited in cleavage and lytic processes took place on the following days. In eggs that ceased to develop and were later expelled at regular intervals, the chorion was separated from choriothete prior to extrusion. The occasional findings that embryogenesis was not interrupted until advanced stages or that normal embryonic and larval development were completed, indicate that treatment doses of 12-13 Krad do not entirely damage the genetic material of the nucleus of sperm, or that repair is possible to some extent. Cyclic changes of the choriothete during pregnancy, its secretion and changes observed during this study, imply that besides facilitating nidation of the egg in the uterus, the choriothete might function in folding of the chorion on the ventral floor of the uterus. (Atomindex citation 12:634792)

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