DNA CONFORMATION IN METHAMOL-ETHANOL-BUFFER

机译：mETHamOL-ETHaNOL-BUFFER中的DNa构象

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DNA in a solvent system consists of roughly equal volumes of methanol and ethanol and 52 buffers has a conservative CD spectrum of very low intensity above 220 nm and an increase of ω258 comparable to that of denatured DNA (about 40%). A direct comparison of this spectrum with the CD of single-stranded DNA reveals many differences, indicating DNA in this solvent system has conformation different from that of denatured DNA. When the alcohols are removed, the B form conformation and normal ω258 are restored in native DNA while single-stranded DNA remains denatured.

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ERNEST KAY;
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年度 1979
页码 1-31
总页数 31
原文格式 PDF
正文语种 eng
中图分类工业技术;
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DNA CONFORMATION IN METHAMOL-ETHANOL-BUFFER

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