首页> 美国政府科技报告 >Comparison of Radiation and Chemical Mutagens with Respect to Protein in Wheat. Part of a Coordinated Programme on the Use of Nuclear Techniques for Seed Protein Improvement. Final Report for the Period 1 February 1971-30 November
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Comparison of Radiation and Chemical Mutagens with Respect to Protein in Wheat. Part of a Coordinated Programme on the Use of Nuclear Techniques for Seed Protein Improvement. Final Report for the Period 1 February 1971-30 November

机译:小麦辐射和化学诱变剂与蛋白质的比较。关于利用核技术促进种子蛋白质改良的协调计划的一部分。 1971年2月1日至11月30日期间的最终报告

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In wheat mutant (M-19) albumins were reduced but globulins and glutelins increased. In opaque-2 mutant of maize, glutelins and albumins increased but the alcohol fraction (zeins) reduced. The amino acid composition of various Osborne protein fractions in maize and wheat was compared. The separation of polypeptides within each Osborne protein fraction was performed, using SDS polyacrylamide gel electrophoresis. Distinct differences in proteins between the normal genotype of maize and opaque-2 mutant was observed. No really lysine-rich polypeptides were found. Relative grain and protein yields were studied in different mutants. Ten mutant lines with increased protein content were crossed with high-yielding varieties. Classes of RNA synthesized on the chromatin were analyzed. The highest ratio of ATP/UTP incorporation was in the class of RNAs which migrated on the gel as mRNA. Radioactivity distribution through the gel proved dependent both on the genotype of chromatin and the origin of the polymerase. (Atomindex citation 11:541173)

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