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首页> 美国政府科技报告 >Identification of Zein Structural Genes in the Maize Genome
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Identification of Zein Structural Genes in the Maize Genome

机译：玉米基因组玉米醇溶蛋白结构基因的鉴定

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Zein is the major storage protein in maize endosperm and is deficient in the amino acids lysine and tryptophan, the amino acids generally limiting in cereal grains. If the two subunits of zein were each encoded by a single gene, it is possible to envisage a program in which altered gene products containing these two amino acids could be selected. Since zein constitutes such a large proportion of the total protein, substitutions of rare amino acids into zein will be reflected in the overall amino acid composition of the kernel. At the moment the authors do not know how many genes for zein there are, nor do they know where they are located in the genome. In the approach to this question the authors have purified the messenger RNAs for the two zein polypeptide chains free from all other species of RNA. The mRNAs are polyadenylated, have a cap structure at their 5' terminus, and contain slightly less than 50% noncoding sequences. Their average length is 1.1 kilobases and they assume they are monocistronic. Using reverse transcriptase and DNA polymerase the authors prepared double-stranded DNA transcripts of the messages. These were inserted into the bacterial plasmid pMB9 and used to transfect E. coli. A number of clones carrying nearly full length inserts have been selected for study. These recombinant DNA molecules are being used as probes to accurately count the number of zein genes in the maize genome, check for amplification, and determine the chromosomal location of the genes. (ERA citation 04:006645)

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