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Studies on the Photoconversion of Protochlorophyllide to Chlorophyllide in Etiolated Plant Material.

机译:黄化植物材料中原叶绿素对叶绿素的光转化研究。

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The synthesis of chlorophyll (Chl) in angiosperms is dependent upon the photochemical conversion of protochlorophyllide (PChlide) to chlorophyllide (Chlide). This study attempts to elucidate the kinetics of the photochemistry,as well as some mechanistic aspects of this reaction and the spectroscopically observable changes that immediately follow it. Experiments were performed on intact leaves,on organelles (etioplasts) extracted from leaves,and on aqueous suspensions of photoactive PChlideprotein complexes (holochrome). Measurement of Chlide fluorescence excited at wavelengths where both Chlide and PChlide absorb, Chlide fluorescence yield,and polarization of Chlide fluorescence,during the photoconversion reaction in holochrome preparations from bean and barley,suggest that the photochemistry is intrinsically first-order,complicated by efficient energy transfer from PChlide to Chlide within aggregated pigment arrays. The fluorescence polarization results also demonstrate that energy transfer occurs among Chlide pigments following photoconversion. Energy transfer occurs effectively in bean holochrome,less effectively in barley extracts,and vanishes in saponin-treated barley extract. Attempts were made to measure photoinduced linear dichroism in bean holochrome,both in dried gelatin films and in solution. The failure to measure such dichroism is most readily explained by the effective depolarization of exciting illumination through energy transfer among aggregated PChlide molecules before photoconversion.

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