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Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions

机译：使用稀释的聚合物溶液毛细管电泳分离DNa限制性片段

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Because the mechanism of DNA separation in capillary electrophoresis is not well understood, selection of polymers is a (open quotes)trial-and-error(close quotes) procedure. We investigated dilute-solution DNA separations by capillary electrophoresis using solutions of four polymers that differ in size, shape and stiffness. Hydroxyethylcellulose of high molecular weight provides excellent separation of large DNA fragments (2027 bp - 23130 bp). Polyvinylpyrrolidone separates DNA from 72 bp to 23 kbp and star-(polyethylene oxide), like linear poly (ethylene oxide), provides separation of fragments up to 1353 bp.

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Braun, B. ; Blanch, W. ; Prausnitz, J. M.;
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年度 1997
页码 1-20
总页数 20
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
DNA; Electrophoresis; Hydroxy Compounds; Polyethylene Glycols; Polymers; Polyvinyls; Separation Processes;

机译：DNa;电泳;羟基化合物;聚乙二醇;聚合物;聚乙烯基;分离过程;

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专利

1. THE USE OF COATED AND UNCOATED CAPILLARIES FOR THE ELECTROPHORETIC SEPARATION OF DNA IN DILUTE POLYMER SOLUTIONS [Review] [J] . Barron AE., Blanch HW., Sunada WM. Electrophoresis: The Official Journal of the International Electrophoresis Society . 1995,第1期

机译：涂覆和未涂覆的毛细管在电泳分离稀聚合物溶液中DNA的使用[综述]
2. Capillary electrophoretic separation of DNA fragments under stepwise changes of polymer solutions [J] . Ming-Feng Huang, Huan-Tsung Chang Instrumentation science & technology: Designs and applications for chemistry, biotechnology, and environmental science . 2000,第5期

机译：逐步改变聚合物溶液的毛细管电泳分离DNA片段
3. Separation of double- and single-stranded DNA restriction fragments: Capillary electrophoresis with polymer solutions under alkaline conditions [J] . Liu Y., Kuhr WG. Analytical chemistry . 1999,第9期

机译：双链和单链DNA限制片段的分离：碱性条件下用聚合物溶液进行毛细管电泳
4. Electrophoretic separation of antisense dna using polymer-solution filled capillary by cross-injection [C] . Shu-Hui Chen, Yu-Cheng Lin, Yu-Hung Chen, Workshop on micro total analysis systems;MTAS'98 . 1998

机译：聚合物溶液填充毛细管通过交叉注入对反义dna进行电泳分离
5. Studies to increase separation selectivity in capillary electrophoretic chiral separations using beta-cyclodextrin-containing polymers. [D] . Cruzado, Ingrid Denisse. 1994

机译：使用含β-环糊精的聚合物提高毛细管电泳手性分离中分离选择性的研究。
6. Free-solution electrophoretic separations of DNA–drag-tag conjugates on glass microchips with no polymer network and no loss of resolution at increased electric field strength [O] . Jennifer Coyne Albrecht, Matthew B. Kerby, Thomas P. Niedringhaus, -1

机译：在玻璃微芯片上的DNA-拖拉标签缀合物的自由溶液电泳分离没有聚合物网络并且在增加电场强度下没有损失
7. Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions [O] . Braun, B., Blanch, W., Prausnitz, J.M. 1997

机译：使用稀释的聚合物溶液毛细管电泳分离DNa限制性片段
8. Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions [R] . Braun, B., Blanch, W., Prausnitz, J. M. 1997

机译：使用稀释的聚合物溶液毛细管电泳分离DNa限制性片段

Capillary electrophoretic separation of DNA restriction fragments using dilute polymer solutions

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