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首页> 美国政府科技报告 >Functionality and Evolutionary History of the Chaperonins in Thermophilic Archaea. A Bioinformatical Perspective
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Functionality and Evolutionary History of the Chaperonins in Thermophilic Archaea. A Bioinformatical Perspective

机译：嗜热古菌中伴侣蛋白的功能和进化历史。生物信息学视角

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We used bioinformatics methods to study phylogenetic relations and differentiation patterns of the archaeal chaperonin 60 kDa heat-shock protein (HSP60) genes in support of the study of differential expression patterns of the three chaperonin genes encoded in Sulfolobus shibatae. Fifty archaeal HSP60 sequences from DNA and protein databases were aligned using the multiple sequence alignment program ITERALIGN (Brocchieri and Karlin, 1998). From this alignment, we identified 397 positions that were used to produce a high-confidence alignment of all sequences. This alignment was used to produce phylogenetic trees by the neighbor-joining (NJ) procedure (Saitou and Nei, 1987) and by the maximum-likelihood (ML) method implemented in the computer program PUZZLE (Strimmer and von Haeseler 1996). In neighbor-joining procedure pair-wise distances between sequences were estimated: (1) inverting SSPA (Significant Segment Painvise Alignment) similarity values (Brocchieri and Karlin, 1998) as suggested by (Feng and Doolittle, 1997); (2) by the transformation of Ota and Nei (1994) based on the Gamma-distribution of the position-dependent mutational rate.

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