首页> 美国政府科技报告 >Developing an Empirically Based Ranking Order for Bone Sampling: Examining the Differential DNA Yield Rates Between Human Skeletal Elements Over Increasing Post Mortem Intervals.
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Developing an Empirically Based Ranking Order for Bone Sampling: Examining the Differential DNA Yield Rates Between Human Skeletal Elements Over Increasing Post Mortem Intervals.

机译:开发一种基于经验的骨采样排序顺序:检测人骨骼元素之间的差异DNa产率,增加死后间隔。

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This study established an empirically based ranking of skeletal elements according to each bones capacity to provide usable genetic material for a DNA identification and assessed how these results varied over longer post mortem intervals (PMI). Currently, selecting skeletal elements for DNA testing is based on the collective wisdom of practitioners who suggest weight-bearing long bones over all other elements. Recent evidence questions the accuracy of this collective wisdom. This study evaluated the differential yield and preservation of DNA by skeletal element in a single individual, replacing intuition with empirically based data to increase the likelihood of successfully generating a DNA profile from skeletal material. A twophase study was conducted to accomplish these goals. Phase 1 entailed analyzing the DNA yield rates of different skeletal elements, from 3 recently skeletonized individuals, to generate an overall rank order from most successful to least successful. Phase 2 was designed to determined if the same elements from Phase 1 were equally successful at longer post mortem intervals (0-3, 4-10, 11-20, and 20+ years) and to give an indication how DNA degradation occurs over time.

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