首页> 美国政府科技报告 >Cloning and Expression of the catA and catBC Gene Clusters from 'Pseudomonas aeruginosa' PAO
【24h】

Cloning and Expression of the catA and catBC Gene Clusters from 'Pseudomonas aeruginosa' PAO

机译:铜绿假单胞菌paO cata和catBC基因簇的克隆与表达

获取原文

摘要

A 9.9-kilobase (kb) BAMIII restriction endonuclease fragment encoding the catA and catBC gene clusters was selected from a gene bank of the Pseudomonas aeruginosa PAO1c chromosome. The catA, catB, and catC genes encode enzymes that catalyze consecutive reactions in the catechol branch of the beta-ketoadipate pathway. A recombinant plasmid, pRo1783, which contains the 9.9-kb BAMIII restriction fragment complemented P. aeruginosa, mutants with lesions in the catA, catB, or catC gene; however, this fragment of chromosomal DNA did not contain any other catabolic gene which had been placed near the catA or catBC cluster based on cotransducibility of the loci. Restriction mapping, deletion subcloning, and complementation analysis showed that the order of the genes on the cloned chromosomal DNA fragment is catA, catB, catC. (Copyright (c) 1988, American Society for Microbiology.)

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号