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Production of Viable 'Giardia' Cysts in vitro: Determination by Fluorogenic Dye Staining, Excystation, and Animal Infectivity in the Mouse and Mongolian Gerbil (Journal Version)

机译：在体外生产活的'贾第虫'囊肿：通过荧光染料染色，脱色和动物传染性测定小鼠和蒙古沙鼠（期刊版）

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The purpose of the research was to document the formation of viable Giardia cysts in vitro. Viability staining, using fluorogenic dyes that required metabolic conversion for detection, and immunocytochemistry at the light microscopic level, provided information on viability as well as the identification of antigens that were similar to those found with in vitro formed cysts. Analysis of cysts formed in vivo and in vitro showed similar morphological appearances by both light and electron microscopy. Cysts formed in vitro were capable of establishing infections in both the mouse and gerbil models for giardiasis. Trophozoites recovered from mice experimentally infected with cysts produced in vitro could be maintained in culture and induced a second time to form cysts in vitro. The model, for the production of viable Giardia cysts in vitro, should facilitate research on controlling the complete life-cycle of Giardia outside an animal host. (Copyright (c) 1988 by the American Gastroenterological Association.)

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Schupp, D. G. ; Januschka, M. M. ; Sherlock, L. A. F. ; Stibbs, H. H. ; Meyer, E. A.;
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年度 1988
页码 1-13
总页数 13
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Giardiasis; Parasitic intestinal diseases; Protozoa; Giardia; Cultured cells; Cysts; Immunohistochemistry; Morphology; Electron microscopy; Laboratory animals; Life cycle; Infectivity; Gerbils; Reprints;

机译：贾第虫病;寄生肠道疾病;原生动物;贾第虫;培养细胞;囊肿;免疫组织化学;形态学;电子显微镜;实验动物;生命周期;传染性;沙鼠;重印;

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1. Comparison of animal infectivity, excystation, and fluorogenic dye as measures of Giardia muris cyst inactivation by ozone. [J] . C W Labatiuk, F W Schaefer, G R Finch, Applied and Environmental Microbiology . 1991,第11期

机译：比较动物的传染性，兴奋性和荧光染料，作为臭氧对贾第鞭毛虫囊肿灭活的量度。
2. Comparison of animal infectivity, excystation, and fluorogenic dye as measures of Giardia muris cyst inactivation by ozone. [J] . C W Labatiuk, F W Schaefer, G R Finch, Applied and Environmental Microbiology . 1991,第11期

机译：比较动物的传染性，兴奋性和荧光染料，作为臭氧对贾第鞭毛虫囊肿灭活的量度。
3. Ethanol and Isopropanol in Concentrations Present in Hand Sanitizers Sharply Reduce Excystation of Giardia and Entamoeba and Eliminate Oral Infectivity of Giardia Cysts in Gerbils [J] . Chatterjee Aparajita, Bandini Giulia, Motari Edwin, Antimicrobial agents and chemotherapy. . 2015,第11期

机译：洗手液中存在的乙醇和异丙醇浓度可显着降低贾第鞭毛虫和Entamoeba的囊泡并消除沙鼠中贾第鞭毛虫囊的口腔感染性
4. The effect of temperature and deposition in reservoir sediments or water on the viability of cultured Giardia lamblia cysts, as determined by fluorogenic dyes. [D] . Romig, Randall Robert. 1990

机译：温度和沉积物沉积物或水中的沉积对培养的贾第鞭毛虫兰氏囊肿生存力的影响，这由荧光染料确定。
5. Comparison of animal infectivity excystation and fluorogenic dye as measures of Giardia muris cyst inactivation by ozone. [O] . C W Labatiuk, F W Schaefer 3rd, G R Finch, 1991

机译：比较动物的传染性兴奋性和荧光染料作为臭氧对贾第鞭毛虫囊肿灭活的量度。
6. Viability of Cryptosporidium parvum oocysts: correlation of in vitro excystation with inclusion or exclusion of fluorogenic vital dyes [O] . A T Campbell, L J Robertson, H V Smith 1992

机译：隐孢子虫卵囊的可行性：含有或排除荧光生气染料的体外切换的相关性
7. Comparison of Animal Infectivity, Excystation, and Fluorogenic Dye as Measures of'Giardia muris' Cyst Inactivation by Ozone [R] . Labatiuk, C. W., Schaefer, F. W., Finch, G. R., 1991

机译：动物感染性，抗生素和荧光染料作为臭氧'消化道'囊肿灭活措施的比较

Production of Viable 'Giardia' Cysts in vitro: Determination by Fluorogenic Dye Staining, Excystation, and Animal Infectivity in the Mouse and Mongolian Gerbil (Journal Version)

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