首页> 美国政府科技报告 >Detection of Environmental Viruses in Sludge: Enhancement of Enterovirus Plaque Assay Titers with 5-Iodo-2'-Deoxyuridine and Comparison to Adenovirus and Coliphage Titers (Journal Version)

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Detection of Environmental Viruses in Sludge: Enhancement of Enterovirus Plaque Assay Titers with 5-Iodo-2'-Deoxyuridine and Comparison to Adenovirus and Coliphage Titers (Journal Version)

机译：污泥中环境病毒的检测：用5-Iodo-2'-Deoxyuridine增强肠道病毒噬斑测定滴度并与腺病毒和大肠杆菌滴度进行比较（期刊版）

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Enteroviruses present in the primary sludge of two wastewater treatment plants were quantitated by plaque assay using a continuous African green monkey kidney cell line (BGM). Incubation of BGM monolayers with 5-iodo-2'-deoxyuridine (50 micrograms/ml) for 4 days prior to use enhanced the number of PFU detected in 10 of 10 concentrated sludge samples. Coliphages also present in these samples were detected using as hosts E. coli C and E. coli A-19, an Hfr strain. E. coli C coliphage titers were consistently higher than E. coli A-19 titers, and were 100-1000 times higher than the enterovirus titers obtained with BGM cells. Adenoviruses present in the sludge samples were detected by immunofluorescence assay. Interestingly, those results suggested that adenoviruses were also more numerous than enteroviruses (as detected by either IDU-treated or untreated BGM plaque assay).

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作者
Williams, F. P. ; Hurst, C. J.;
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年度 1988
页码 1-7
总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Enteroviruses; Sewage sludge; Adenoviruses; Coliphage; Detection; Iodine organic compounds; Reprints;

机译：肠道病毒;污水污泥;腺病毒;大肠杆菌;检测;碘有机化合物;重印;

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专利

1. Evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses. [J] . W H Benton, C J Hurst Applied and Environmental Microbiology . 1986,第5期

机译：在人类肠道病毒的噬菌斑滴度测定中评估混合细胞类型和5-碘-2'-脱氧尿苷处理。
2. Mumps Virus-Specific Antibody Titers from Pre-Vaccine Era Sera: Comparison of the Plaque Reduction Neutralization Assay and Enzyme Immunoassays [J] . Jeremy Mauldin, Kathryn Carbone, Henry Hsu, Journal of Clinical Microbiology . 2005,第9期

机译：疫苗前时代流行性腮腺炎病毒特异性抗体滴度：噬菌斑减少中和测定和酶免疫测定的比较
3. Comparison of PCR and plaque assay for detection and enumeration of coliphage in polluted marine waters. [J] . J B Rose, X Zhou, D W Griffin, Applied and Environmental Microbiology . 1997,第11期

机译：PCR和噬菌斑检测法在污染海水中检测和计数噬菌体的比较。
4. Evaluation of mixed cell types and 5-iodo-2-deoxyuridine treatment upon plaque assay titers of human enteric viruses. [O] . W H Benton, C J Hurst 1986

机译：人肠病毒的噬菌斑滴定度评估混合细胞类型和5-碘-2-脱氧尿苷处理。
5. Mumps Virus-Specific Antibody Titers from Pre-Vaccine Era Sera: Comparison of the Plaque Reduction Neutralization Assay and Enzyme Immunoassays [O] . Mauldin, Jeremy, Carbone, Kathryn, Hsu, Henry, 2005

机译：疫苗前时代流行性腮腺炎病毒特异性抗体滴度：噬菌斑减少中和测定和酶免疫测定的比较
6. Evaluation of Mixed Cell Types and 5-Iodo-2'-Deoxyuridine Treatment upon Plaque Assay Titers of Human Enteric Viruses (Journal Version) [R] . Benton, W. H. , Hurst, C. J. 1986

机译：评估混合细胞类型和5-Iodo-2'-Deoxyuridine治疗人类肠道病毒的噬斑测定滴度（期刊版）

Detection of Environmental Viruses in Sludge: Enhancement of Enterovirus Plaque Assay Titers with 5-Iodo-2'-Deoxyuridine and Comparison to Adenovirus and Coliphage Titers (Journal Version)

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