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In-Depth Survey Report: Control Technology Assessment of Enzyme Fermentation Processes at Gist-Brocades USA, Incorporated, Kingstree, South Carolina

机译:深入调查报告:南卡罗来纳州金斯特里Gist-Brocades Usa,Incorporated的酶发酵过程控制技术评估

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The controls and containment capabilities of the proteolytic enzyme manufacturing process was examined at the Gist-Brocades USA, Inc., facility (SIC-2869) located in Kingstree, South Carolina, where 160 workers were involved in the production of the industrial enzyme protease (9001927) using a microbial strain of Bacillus-subtilis. A comparison between viable sample concentrations at selected sites and background levels indicated that most emissions occur at high energy operations including the agitator shafts, belt conveyor, filter press, and sampling ports. Exhaust gases from the fermentor and seed tanks were also sites of increased emission levels, as the water scrubber did not completely control the release of entrained production microorganisms. During removal of the filter cake from the filter press, the microbial levels ranged as high as 28990 colony forming units/cubic meter in a single sample. In all but one location of the enzyme recovery area, acetone (67641) levels were less than 0.01/cc m. Emissions from sampling ports could be lowered by following the company procedure for opening the steam valve.

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