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Methods of Unidirectional, Site-Specific Integration Into a Genome, Compositions and Kits for Practicing the Same.

机译：单向，位点特异性整合到基因组中的方法，用于实践相同的组合物和试剂盒。

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摘要

The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided system includes a site-specific integrating expression cassette (INTEC) vector, consisting of (a) a polynucleotide of interest operably linked to a promoter, (b) a single recombination site, and (c) a hybrid recombination site. In using the subject systems for site-specific integration, the INTEC vector and integrase are introduced into the target cell and the cell is maintained under conditions sufficient to provide for site-specific integration of the nucleic acid into the target cell genome via a recombination event mediated by the site-specific recombinase. Also provided are kits that include the subject systems. The subjects systems, methods and kits find use in a variety of different applications, several representative ones of which are described in detail as well.

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作者
Calos, M. P.;
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年度 2005
页码 1-169
总页数 169
原文格式 PDF
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关键词
Patent applications; Nucleic acid integration; Genome; Target cells; Systems; Methods; Compositions; Unidirectional; Cells(Biology); Polynucleotides; Recombination sites; Kits; Unidirectional site-specific integration system; Site-specific integrating expression casse;

机译：专利申请;核酸整合;基因组;靶细胞;系统;方法;组合物;单向;细胞（生物学）;多核苷酸;重组位点;试剂盒;单向位点特异性整合系统;位点特异性整合表达casse;

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Methods of Unidirectional, Site-Specific Integration Into a Genome, Compositions and Kits for Practicing the Same.

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