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首页> 美国政府科技报告 >Fate and Transport of Recombinant 'Sinorhizobium meliloti' with Enhanced N2-211 Fixing Abilities during a Multiyear Field Trial with Transgenic Alfalfa
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Fate and Transport of Recombinant 'Sinorhizobium meliloti' with Enhanced N2-211 Fixing Abilities during a Multiyear Field Trial with Transgenic Alfalfa

机译：转基因苜蓿多年田间试验中具有增强N2-211固定能力的重组'sinorhizobium meliloti'的命运和运输

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摘要

A field test with transgenic alfalfa and recombinant Sinorhizobium meliloti was211u001eperformed in 1995/96 in Corvallis, Oregon. Two additional years of monitoring the 211u001efate of S. meliloti occurred after the aflalfa plants were sprayed. Parental and 211u001etwo transgenic lines (alpha amylase or lignin peroxidase producing) of alfalfa 211u001ewere either not inoculated or inoculated with parental S. meliloti strain RMBPC, 211u001eantibiotic-resistant strain RMB7201, or antibiotic-resistant and enhanced 211u001enitrogen-fixing strain RMBPC-2. Over two growing seasons, plant and bulk soil 211u001esamples were obtained from triplicate plots of the 12 treatments. Selective 211u001eplating, colony hybridization, and PCR methodologies were used to identify 211u001erecombinant inocula recovered from the field. During the first year, only 211u001einoculated rows tested positive for recombinant S. meliloti with counts up to 5.6 211u001ex 10(sup 4) CFU/g dry wt soil(gdws). In the second year, counts increased in 211u001einoculated rows by about an order of magnitude to up to 8.7 x 10(sup 5) CFU/gdws. 211u001eAfter unusual flooding of the plot in the winter of 1996, the recominant S. 211u001emeliloti also became detectable in the bulk soil surrounding uninoculated alfalfa 211u001eplants across the field plot.

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