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Determination of Ochratoxin A in Grain by Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay.

机译：单克隆抗体酶联免疫吸附法测定谷物中赭曲霉毒素a的含量。

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The simple rapid and sensitive enzyme-linked immunosorbent assay (ELISA) methods, direct and indirect ELISA, for quantitation of ochratoxin A in cereal had been developed by the utilization of monoclonal antibody on immunomicroplate. Direct ELIAS was found to be less time consuming than indirect ELISA. For direct ELISA, recovery of 1-500 parts per billion (ppb) OA added to wheat was 78.9-100.0% and rice was 88.9-120.0%. For indirect ELISA, recovery of 1-500 ppb OA added to wheat was 79.0-110.0% and rice was 82.0-120.0%. The minimal detection level for OA was 1 ppb. Analyses of 31 samples that caused humanintoxicant for OA showed that the ELISA results agreed well with those obtained by thin-layer chromatography.

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Yang, C.; Luo, X.; Liu, C.; Li, W.; Li, Y.; Zhao, D.; Ji, R.;
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年度 1994
页码 1-9
总页数 9
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Grains(Food); Ochratoxins; Assaying; Test methods; Chemical analysis; Toxicology; Monoclonal antibodies; Carcinogenesis; Neoplasms; Wheat; Rice; Foreign technology; ELISA(Enzyme-linked immunosorbent assay); Enzyme-linked immunosorbent assay; Ochratoxin A;

机译：谷物（食品）;赭曲霉毒素;测定;测试方法;化学分析;毒理学;单克隆抗体;癌变;肿瘤;小麦;水稻;国外技术; ELIsa（酶联免疫吸附测定）;酶联免疫吸附测定;赭曲霉毒素a;

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Determination of Ochratoxin A in Grain by Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay.

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