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首页> 外文OA文献 >Morphological, Physiological and Metabolomic Response of transgenic tobacco plants (N. tabacum L.)overexpressing GmGSTU4 under Drought Stress
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Morphological, Physiological and Metabolomic Response of transgenic tobacco plants (N. tabacum L.)overexpressing GmGSTU4 under Drought Stress

机译：转基因烟草（N.tabacum L.）的形态学，生理学和代谢组学反应在干旱胁迫下过表达GmGsTU4

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GSTs appear to have a significant role in plants’ adaptation under abiotic stress as many isoenzymes are found to be differentially expressed under these conditions yet, little is known about the regulatory functions of GSTs. Wild type and transgenic tobacco plants over-expressing the soybean GmGSTU4 of cultivars Basmas, Burley and Virginia were grown in vitro under 100 and 200mM mannitol or in soil (plant pots) by withholding watering for 15 days. However, GmGSTU4 plants did not exhibit significantdifferences in drought tolerance compared to wild-type plants. Morphological (shoot length, total and root fresh weight) and physiological (chlorophyll content, relative water content and photosynthetic capacity) parameters of transgenic plants did not differ from the wild-type in the presence of 100 or 200mM mannitol or in the soil when watering was halted. Metabolite profiling was used to understand the dynamics between the wild-type and transgenic tobacco response to drought stress. Different metabolic pathways are involved in production of osmoprotectants. These molecules accumulate in plants under stress conditions as adaptive mechanism, which can provide stress tolerance. GmGSTU4 plants did not exhibit difference in drought tolerance compared to wild-type plants, however metabolomics analysis indicated alterations in metabolite profile and increased concentration of sorbitol, glycerol and pyruvic acid. In conclusion, overexpression of GmGSTU4 in transgenic plants did notaffect their drought stress tolerance although it has altered their metabolite profile possible because of diverse effects on plant stress tolerance mechanism.
机译：在非生物胁迫下，GST似乎在植物适应中起着重要作用，因为发现许多同工酶在这些条件下会差异表达，但对GST的调节功能知之甚少。过量表达Basmas，Burley和Virginia大豆GmGSTU4的野生型和转基因烟草植物在100和200mM甘露醇下或在土壤（花盆）中通过禁水15天在体外生长。然而，与野生型植物相比，GmGSTU4植物在耐旱性上没有表现出显着差异。在存在100或200mM甘露醇的情况下或浇水时，转基因植物的形态学参数（茎长，总重和根鲜重）和生理学参数（叶绿素含量，相对水分含量和光合能力）与野生型没有区别。被停止了。代谢物谱用于了解野生型和转基因烟草对干旱胁迫的反应之间的动态关系。渗透保护剂的产生涉及不同的代谢途径。这些分子作为适应性机制在胁迫条件下在植物中积累，可以提供胁迫耐受性。与野生型植物相比，GmGSTU4植物的耐旱性没有差异，但是代谢组学分析表明，代谢物谱发生了变化，山梨醇，甘油和丙酮酸的浓度增加。总之，GmGSTU4在转基因植物中的过表达并不影响其耐旱性，尽管由于对植物耐逆性机制的多种影响而改变了其代谢产物的分布。

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