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首页> 外文OA文献 >Efficient Extraction of Anti-Inflammatory Active Ingredients from Schefflera octophylla Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with HPLC
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Efficient Extraction of Anti-Inflammatory Active Ingredients from Schefflera octophylla Leaves Using Ionic Liquid-Based Ultrasonic-Assisted Extraction Coupled with HPLC

机译：使用基于离子液体的超声辅助提取与HPLC耦合的离子液体超声波辅助提取有效提取抗炎活性成分叶片叶片

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摘要

Schefflera octophylla (Lour.) Harms, a kind of traditional Chinese medicine (TCM), is commonly used for anti-inflammatory, analgesic, rheumatism, fever, and hemostasis therapy. In our previous studies, two major triterpenoids were isolated and identified from leaves of S. octophylla, and evaluated for their inhibitory effects on lipopolysaccharide (LPS)-induced nitric oxide production in RAW264.7 cells; both of them displayed significant anti-inflammatory activities at their noncytotoxic concentrations. Therefore, it is very useful to establish an efficient and green extraction method to isolated the two major triterpenoids from leaves of S. octophylla. In this paper, ionic liquid based ultrasonic-assisted extraction (ILUAE) was successfully applied to extract the two major triterpenoids from leaves of S. octophylla. Four single factors (ionic liquids (ILs) concentration, solid−liquid ratio, centrifugal speed, mesh number), with a greater impact on extraction rate, were selected from a variety of influencing factors, and the optimal conditions were obtained by Box−Behnken response surface methodology (RSM). Under optimal conditions, the total extraction yield and extraction rate of two triterpenoids were 288.03 mg/g and 28.80%, respectively, which was 6.80% higher than that of 70% Ethanol (220 mg/g and 22%, respectively).
机译：鸭脚木（阴沉。）危害，一种中国的传统医学（TCM）的，通常用于消炎，止痛，风湿，发热和止血治疗。在我们以前的研究，两个主要的三萜类化合物的分离和来自酿酒柴叶识别，并评价对脂多糖（LPS）的抑制作用诱导在RAW264.7细胞中一氧化氮的产生;他们都在他们的无细胞毒性浓度显示显著抗炎活性。因此，它是非常有用的建立高效绿色提取方法从S.柴的叶中分离的两个主要的三萜类化合物。在本文中，离子液体基于超声波的辅助提取（ILUAE）成功地应用于来自酿酒柴叶提取两个主要三萜类化合物。四个单因素（离子液体（离子液体）浓度，固液比，离心速度，网数），对提取率的影响更大，从各种影响因素的选择，并且由箱Behnken法获得的最佳条件响应面分析法（RSM）。在最佳条件下，二三萜类化合物的总提取率为占和提取率分别为288.03 mg / g和28.80％，分别明显高于70％的乙醇（220毫克/克和22％，分别地）的更高的6.80％。

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