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首页> 外文OA文献 >Combined vaccine regimen based on parenteral priming with a DNA vaccine and administration of an oral booster consisting of a recombinant Salmonella enterica serovar typhimurium vaccine strain for immunization against infection with human-derived enterotoxigenic Escherichia coli strains
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Combined vaccine regimen based on parenteral priming with a DNA vaccine and administration of an oral booster consisting of a recombinant Salmonella enterica serovar typhimurium vaccine strain for immunization against infection with human-derived enterotoxigenic Escherichia coli strains

机译：基于肠胃外引物的联合疫苗方案与DNA疫苗的组合，并给予由重组沙门氏菌血清型鼠伤寒沙门氏菌疫苗菌株组成的口服加强疫苗，以免疫人源性产肠毒素的大肠杆菌菌株

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Repeated evidence has demonstrated that combined primer-booster immunization regimens can improve both secreted and humoral immune responses to antigens derived from viral, bacterial, and parasitic pathogens. for the present work, we evaluated the synergic serum immunoglobulin G (IgG) and fecal IgA antibody responses elicited in BALB/c mice who were intramuscularly primed with a DNA vaccine, pRECFA, followed by oral boosting with an attenuated Salmonella enterica serovar Typhimurium vaccine (HG3) strain, with both vaccines encoding the structural subunit (CfaB) of the CFA/I fimbriae produced by human-derived enterotoxigenic Escherichia coli (ETEC) strains. the immunological properties of the vaccine regimen were evaluated according to the order of the administered vaccines, the nature of the oral antigen carrier, the age of the vaccinated animals, the interval between the priming and boosting doses, and the amount of injected DNA. the production of gamma interferon and the IgG2a subclass in serum indicated that mice immunized with the primer-booster regimen developed prevailing type 1 T-cell-dependent immune responses. the synergic effect of the vaccine regimen on the induced antibody responses was also revealed by its ability to block the adhesive properties of CFA/I fimbriae expressed by live bacteria, as shown by the inhibition of Caco-2 cell and human erythrocyte binding. Moreover, DBA2 newborn mice were protected from lethal challenges with a CFA/I+ ETEC strain after the incubation of live bacteria with serum samples harvested from mice who were subjected to the primer-booster regimen. We propose, therefore, that the DNA primer-Salmonella booster regimen represents an alternative for the development of vaccines requiring both mucosal and systemic antibody responses for immunological protection.
机译：反复的证据表明，组合的引物-加强免疫方案可以改善对病毒，细菌和寄生性病原体抗原的分泌和体液免疫反应。在目前的工作中，我们评估了用DNA疫苗pRECFA肌肉内接种的BALB / c小鼠引起的协同血清免疫球蛋白G（IgG）和粪便IgA抗体反应，然后口服减毒沙门氏菌血清型鼠伤寒疫苗加强免疫（ HG3）菌株，两种疫苗均编码人源肠毒素性大肠杆菌（ETEC）菌株产生的CFA / I菌毛的结构亚基（CfaB）。根据接种疫苗的顺序，口服抗原载体的性质，接种动物的年龄，初次和加强剂量之间的间隔以及注射的DNA量评估疫苗方案的免疫学特性。血清中γ干扰素和IgG2a亚类的产生表明，用引物-升压方案免疫的小鼠产生了主要的1型T细胞依赖性免疫应答。疫苗方案对诱导的抗体应答的协同作用还通过其阻断活细菌表达的CFA / I菌毛的粘附特性的能力得以揭示，如对Caco-2细胞和人红细胞结合的抑制所表明的。此外，在将活细菌与从接受了引物-加强剂疗法的小鼠收集的血清样品孵育后，用CFA / I + ETEC菌株保护了DBA2新生小鼠免受致命攻击。因此，我们建议DNA引物-沙门氏菌加强免疫方案代表了一种开发疫苗的替代方法，该疫苗既需要粘膜抗体也需要全身抗体，以进行免疫保护。

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