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首页> 外文OA文献 >Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo
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Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo

机译：桐城猪与大白猪在体内桐城猪和大白猪之间替代剪接差异的全局分析

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Alternative splicing (AS) plays a significant role in regulating gene expression at the transcriptional level in eukaryotes. Flexibility and diversity of transcriptome and proteome can be significantly increased through alternative splicing of genes. In the present study, transcriptome data of peripheral immune organs including spleen and inguinal lymph nodes (ILN) were used to identify AS difference between PRRSV-resistant Tongcheng (TC) pigs and PRRSV-susceptible Large White (LW) pigs artificially infected with porcine reproductive and respiratory syndrome virus (PRRSV) in vivo. The results showed that PRRSV infection induced global alternative splicing events (ASEs) with different modes. Among them, 373 genes and 595 genes in the spleen and ILN of TC pigs, while 458 genes and 560 genes in the spleen and ILN of LW pigs had significantly differential ASEs. Alternative splicing was subject to tissue-specific and lineage-specific regulation in response to PRRSV infection. Enriched GO terms and pathways showed that genes with differential ASEs played important roles in transcriptional regulation, immune response, metabolism, and apoptosis. Furthermore, a splicing factor associated with apoptosis, SRSF4, was significantly upregulated in LW pigs. Functional analysis on apoptosis associated genes was validated by RT-PCR and DNA sequencing. These findings revealed different response to PRRSV between PRRSV-resistant TC pigs and PRRSV-susceptible LW pigs at the level of alternative splicing, suggesting the potential relationship between AS and disease resistance to PRRSV.
机译：选择性剪接（AS）起着在真核生物转录水平调节基因表达一个显著作用。转录组和蛋白质组的灵活性和多样性可通过基因的选择性剪接来显著增加。在本研究中，外周免疫器官包括脾和腹股沟淋巴结（ILN）的转录组数据被用来确定AS PRRSV耐桐城（TC）猪和PRRSV易感大白猪（LW）的猪人工感染猪生殖之间差与呼吸综合征病毒（PRRSV）的体内。结果表明，PRRSV感染引起的全球另类剪接事件系统（ASES）具有不同的模式。其中，373个基因和595个基因在TC猪的脾和ILN，而458个基因和560个基因在脾脏LW猪ILN和有显著差的ASE。可变剪接有待组织特异性和谱系特异性调节响应于PRRSV感染。富集的GO术语和途径表明，基因与差的ASE转录调节，免疫反应，代谢和细胞凋亡中发挥了重要的作用。此外，与细胞凋亡，SRSF4相关联的剪接因子，在LW猪中显著上调。对细胞凋亡相关基因的功能分析通过RT-PCR和DNA测序验证。这些发现揭示了对PRRSV PRRSV耐TC猪和PRRSV易感LW猪之间的选择性剪接的水平不同的响应，这表明对PRRSV AS和抗病性之间的电势关系。

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