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首页> 外文OA文献 >One-step purification of murine IgM and human α2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin
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One-step purification of murine IgM and human α2-macroglobulin by affinity chromatography on immobilized snowdrop bulb lectin

机译：鼠IgM和人α2-大胶质蛋白的一步纯化通过亲和层析在固定化的旱蛋白凝集素上

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A new mannose-specific plant lectin (GNA) isolated from the snowdrop bulb was immobilized on Sepharose 4B and employed for the purification of certain glycoproteins with high-mannose type glycan chains. Murine IgM bound tightly to this column and was eluted with 0.1 methyl [alpha]--mannoside whereas bovine and murine IgG were not bound. When a murine hybridoma serum containing IgM monoclonal antibody was applied to this column, highly purified IgM antibody was obtained after elution with methyl [alpha]--mannoside. On the contrary, human IgM was not bound by this column despite reports that it contains high-mannose type glycan chains. [alpha]2-Macroglobulin was the sole glycoprotein present in human serum which was bound by the immobilized snowdrop lectin column. It appears that only glycoproteins containing multiple Man-([alpha]1,3)Man units are bound to the immobilized lectin.
机译：将从Snowdrop灯泡分离的新的甘露糖特异性植物凝集素（GNA）固定在Sepharose 4b上，并用于用高甘露糖型聚糖链纯化某些糖蛋白。鼠IgM紧紧地绑定到该柱中，用0.1甲基α-甘露糖苷洗脱，而牛和鼠IgG未结合。当含有含有IgM单克隆抗体的鼠杂交瘤血清施加到该塔时，用甲基α-甘露糖苷洗脱后获得高度纯化的IgM抗体。相反，尽管报告其含有高甘露糖型甘草链，但是本栏的人IgM不受约束。 [α] 2-甲基蟾蛋白是存在于人血清中的唯一糖蛋白，其由固定的Snowdrop凝集素柱结合。似乎只有含有多种人 - （α1,3）人单元的糖蛋白与固定的凝集素结合。

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