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Determination of major anthocyanin pigments and flavonols in red grape skin of some table grape varieties (Vitis vinifera sp.) by high-performance liquid chromatography–photodiode array detection (HPLC-DAD)

机译:用高效液相色谱 - 光电二极管阵列检测(HPLC-DAD),测定一些表葡萄品种红葡萄皮肤和黄葡萄皮肤的红葡萄皮肤和黄酮酚(

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摘要

Aim: The aim of this study was the investigation of the anthocyanin and flavonol content in grape skin extracts. Five prevalent anthocyanin-types and four flavonol-types were determined in the skin of three red table grape varieties widely cultivated in ElTarf (Algeria). Methods and results: The identification of the compounds was performed by HPLC-DAD based on C-18 reversed phase column separation. Results from HPLC analysis showed that malvidin and petunidin-3-O-glucoside were the major anthocyanin glucoside, whereas quercetin-3-O-glucoside was the major flavonol among the four identified. Conclusion: The content of anthocyanins and flavonols in the grape skin of three grapevine (Vitis vinifera) varieties exhibits notable differences among the cultivars studied, confirming their importance in the varietal characterization. The highest concentrations of total anthocyanins and flavonols corresponded to the Gros noir variety. The results of the present study also indicate that the grape skin extracts of these Algerian cultivars can be used as easily accessible source of natural antioxidants. Significance and impact of the study: To the best of our knowledge, this is the first report on the identification of different anthocyanins and flavonols in berry skin from some red grape varieties largely cultivated in this region of Algeria.
机译:目的:本研究的目的是调查葡萄皮肤提取物中的花青素和黄酮醇含量。在Eltarf(阿尔及利亚)广泛种植的三个红色表葡萄品种的皮肤中,确定了五种普遍的花青素型和四种黄酮型。方法和结果:基于C-18反相柱分离,通过HPLC-DAD进行化合物的鉴定。 HPLC分析结果表明,麦类和喇叭蛋白-3-O-葡糖苷是主要的花青素葡糖苷,而槲皮素-3-O-葡糖苷是四种鉴定的主要黄酮。结论:三种葡萄葡萄酒(葡萄藻)品种葡萄皮中的花青素和黄酮醇的含量在研究中表现出显着的差异,证实了它们在品种表征中的重要性。浓度最高浓度的花青素和黄酮醇对应于GROS Noir品种。本研究的结果还表明,这些阿尔及利亚品种的葡萄皮肤提取物可用作易于进入的天然抗氧化剂来源。这项研究的意义和影响:据我们所知,这是一份关于在阿尔及利亚该地区大部分栽培的浆果皮肤鉴定不同花药和黄酮类药物的第一份报告。

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