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首页> 外文OA文献 >Integrated analysis of mRNA-seq and miRNA-seq in calyx abscission zone of Korla fragrant pear involved in calyx persistence
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Integrated analysis of mRNA-seq and miRNA-seq in calyx abscission zone of Korla fragrant pear involved in calyx persistence

机译：涉及Calyx持久性的Korla芳香梨花菌脱落区mRNA-SEQ和miRNA-SEQ的综合分析

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Abstract Background The objective of this study was to characterize molecular mechanism of calyx persistence in Korla fragrant pear by transcriptome and small RNA sequencing. Abscission zone tissues of flowers at three stages (the first, fifth and ninth days of the late bloom stage), with 50 mg/L GA3 (calyx persistence treatment, C_1, C_5, C_9) or 500 mg/L PP333 (calyx abscission treatment, T_1, T_5, T_9), were collected and simultaneously conducted transcriptome and small RNA sequencing. Results Through association analysis of transcriptome and small RNA sequencing, mRNA-miRNA network was conducted. Compared calyx persistence groups with calyx abscission groups during the same stage, 145, 56 and 150 mRNA-miRNA pairs were obtained in C_1 vs T_1, C_5 vs T_5 and C_9 vs T_9, respectively; When C_1 compared with C_5 and C_9, 90 and 506 mRNA-miRNA pairs were screened respectively, and 255 mRNA-miRNA pairs were obtained from the comparison between C_5 and C_9; When T_1 compared with the T_5 and T_9, respectively, 206 and 796 mRNA-miRNA pairs were obtained, and 383 mRNA-miRNA pairs were obtained from the comparison between T_5 and T_9. These mRNAs in miRNA-mRNA pairs were significantly enriched into the terpenoid backbone biosynthesis, photosynthesis - antenna proteins, porphyrin and chlorophyll metabolism, carotenoid biosynthesis, zeatin biosynthesis and plant hormone signal transduction. In addition, we obtained some key genes from miRNA-mRNA pairs that may be associated with calyx abscission, including protein phosphatase 2C (psi-miR394a-HAB1), receptor-like protein kinase (psi-miR396a-5p-HERK1), cellulose synthase-like protein D3 (psi-miR827-CSLD3), beta-galactosidase (psi-miR858b-β-galactosidase), SPL-psi-miR156j/157d, abscisic acid 8′-hydroxylase 1 (psi-miR396a-5p-CYP707A1) and auxin response factor (psi-miR160a-3p-ARF6, psi-miR167d-ARF18, psi-miR167a-5p-ARF25), etc. Conclusion By integrated analysis mRNA and miRNA, our study gives a better understanding of the important genes and regulation pathway related to calyx abscission in Korla fragrant pear. We have also established the network of miRNA-mRNA pairs to learn about precise regulation of miRNA on calyx abscission.
机译：摘要背景本研究的目的是通过转录组和小RNA测序来表征的花萼持续在库尔勒香梨的分子机制。在三个阶段的花离区组织（晚期开花阶段的第一，第五和第九天），用50mg / L GA3（花萼持久性治疗，C_1，C_5，C_9）或500 mg / L的PP333（花萼脱落治疗，T_1，T_5，T_9），收集和同时进行转录组和小RNA测序。结果通过转录组和小RNA测序的关联分析，mRNA表达-miRNA的网络中进行的。在C_1得到VS T_1，C_5 VS T_5和C_9 VS T_9，分别比较花萼持久性基团与花萼脱落基团在相同的阶段，145，56和150的mRNA-miRNA对;当C_1与C_5和C_9，90和506的mRNA-miRNA对分别筛选，从C_5和C_9之间的比较获得的mRNA 255体miRNA对比较;当T_1与T_5和T_9相比，分别获得206和796的mRNA-miRNA的对，并从T_5和T_9之间的比较获得的mRNA 383体miRNA对。这些mRNA在miRNA的体mRNA对被显著富集到骨干萜的生物合成，光合作用 - 天线蛋白质，卟啉，叶绿素代谢，类胡萝卜素生物合成，玉米素生物合成和植物激素信号转导。此外，我们获得从的miRNA-mRNA的对一些关键基因可与花萼脱落相关联，包括蛋白磷酸酶2C（PSI-miR394a-HAB1），类受体蛋白激酶（PSI-miR396a-5P-HERK1），纤维素合酶样蛋白D3（PSI-miR827-CSLD3），β-半乳糖苷酶（PSI-miR858b-β半乳糖苷酶），SPL-PSI-miR156j / 157D，脱落酸8'-羟化酶1（PSI-miR396a-5P-CYP707A1）和生长素应答因子（PSI-miR160a-3P-ARF6，PSI-miR167d-ARF18，PSI-miR167a-5P-ARF25）等结论通过综合分析的mRNA和miRNA，我们的研究提供了一个更好的重要基因和调控途径了解在库尔勒香梨与花萼脱落。我们还建立的miRNA表达对网络了解的花萼脱落的miRNA的精确调节。

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