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首页> 外文OA文献 >Inhibitory Effect of Two Traditional Chinese Medicine Monomers, Berberine and Matrine, on the Quorum Sensing System of Antimicrobial-Resistant Escherichia coli
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Inhibitory Effect of Two Traditional Chinese Medicine Monomers, Berberine and Matrine, on the Quorum Sensing System of Antimicrobial-Resistant Escherichia coli

机译：两种中药单体，小檗碱和苦参碱的抑制作用，抗菌抗菌大肠杆菌批量传感系统

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The quorum sensing (QS) system controls bacterial biofilm formation, which is highly related to the virulence and resistance of pathogens. In the present study, the effect of two traditional Chinese medicine (TCM) monomers, berberine and matrine, on biofilm formation and QS-related gene expression of antimicrobial-resistant (AMR) Escherichia coli strains was investigated by laser scanning confocal microscopy (LSCM) observation and real-time PCR. The results indicated a roughly positive relationship between biofilm formation ability and antimicrobial resistance. LSCM observation showed that berberine and matrine inhibited biofilm formation of AMR E. coli strains at 1/2 minimal inhibitory concentration (MIC) (1/2 MIC berberine at OD630: 0.1020; 1/2 MIC matrine: OD630: 0.1045); furthermore, abnormal cell morphology such as rounded and elongated cells was also observed. This finding was consistent with the downregulation of QS-related genes: luxS, pfS, sdiA, hflX, motA, and fliA. At 1/2 MIC and 1/4 MIC concentrations of berberine, a significant downregulation of luxS, pfS, hflX, ftsQ, and ftsE was observed. The results indicate that berberine and matrine can inhibit biofilm formation by inhibiting the QS system and that berberine is more effective than matrine.
机译：仲裁传感（QS）系统控制细菌生物膜形成，这与病原体的毒力和抗性有高度相关。在目前的研究中，通过激光扫描共聚焦显微镜（LSCM）研究了两种中药（TCM）单体，小檗碱和苦参碱对生物膜形成和QS相关基因表达的抗菌抗菌（AMR）大肠杆菌菌株的影响观察和实时PCR。结果表明了生物膜形成能力与抗微生物抗性之间的大致阳性关系。 LSCM观察表明，小檗碱和苦参碱在1/2最小抑制浓度（MIC）（OD630的1/2 MIC Berberine）（OD630：0.1020; 1/2麦克白核：OD630：0.1045）中抑制了AMR大肠杆菌菌株的生物膜形成。此外，还观察到诸如圆形和细胞细胞的异常细胞形态。这一发现与QS相关基因的下调：LUX，PFS，SDIA，HFLX，MOTA和FLIA的下调。在1/2麦克风和1/4麦克风浓度下，观察到Lux，PFS，HFLX，FTSQ和FTSE的显着下调。结果表明，小檗碱和苦参碱可以通过抑制QS系统而抑制生物膜形成，并且小檗碱比苦参碱更有效。

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