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Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors

机译:体积氧转移系数(KLA)对荷兰氏菌尼尔·瓦斯·瓦斯·瓦斯的批量生产的影响和内环空运生物反应器的影响

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摘要

Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000 U l−1; kLa = 30 h−1), while the β-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same kLa value (12 h−1) in STB and ALB, the xylanase and β-xylosidase productions were higher in ALB (7000 U l−1 xylanase; 2100 U l−1 β-xylosidase) than STB (4500 U l−1 xylanase; 1800 U l−1 β-xylosidase).
机译:氧合是在混合缓冲生物反应器的设计和操作中涉及的重要参数,并且可以通过氧气传质系数(KLA)分析。在本研究中,在搅拌釜生物反应器(STB)和空运生物反应器(ALB)的分批发酵在一系列KLA值下操作,试图优化和比较由真菌曲霉群组合成的细胞外木聚糖酶和β-木糖苷酶的活性Tieghem,使用Corncob作为诱导源。在STB(9000 U L-1; KLA = 30H-1)上观察到较高的木聚糖酶水平,而β-木质酶产生在两个生物反应器中相似。然而,当在STB和ALB中的相同KLA值(12h-1)比较酶活性时,溴酶和β-木糖苷酶生产中的alAl(7000u l-1木聚糖酶; 2100 U L-1β- Xylosidase)比STB(4500u L-1木聚糖酶; 1800 U L-1β-木糖苷酶)。

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