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首页> 外文OA文献 >Development of multiple interactions mixed matrix membrane chromatography using lewatit MP500 anion resin and lewatit CNP 105 cation resin for whey protein fractionation
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Development of multiple interactions mixed matrix membrane chromatography using lewatit MP500 anion resin and lewatit CNP 105 cation resin for whey protein fractionation

机译：用lewatit MP500阴离子树脂和lewatit CNP 105阳离子树脂进行多重相互作用混合基质膜色谱分离乳清蛋白的研究

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摘要

The conventional method to purify protein is by using packed bed column chromatography. However, this method had several limitations such as high pressure drop and limited flow rate operation. Membrane chromatography can be used to overcome the limitation of packed column but the preparation of adsorptive membrane requires harsh chemical modifications. Mixed matrix membrane (MMM) preparation concept can be used as an alternative route to prepare membrane chromatography by physical blending of adsorptive resin with membrane polymer solution. In the current research, multiple interactions MMM chromatography was developed for whey protein fractionation using 7.5 wt% CNP105 cation resin and 42.5 wt% MP500 anion resin relative to base polymer content. The resins were blend at different composition in EVAL and cellulose base polymer matrix. Based on HPLC and SDS-PAGE analysis, both acidic and basic whey proteins were bound to the multiple interactions MMM in single run of whey batch fractionation. The binding capacity for major acidic whey proteins using EVAL based MMM are 4.255 mg BSA bound/ g MMM, 60.887 mg α-Lac bound/ g MMM and 231.788 mg β-Lac/ g MMM. For CA based MMM, the binding capacity are 2.970 mg BSA bound/ g MMM, 42.392 mg α-Lac bound/ g MMM and 179.817 mg β-Lac/ g MMM.
机译：纯化蛋白质的常规方法是使用填充床柱色谱法。但是，这种方法有一些局限性，例如高压降和有限的流速操作。膜色谱法可用于克服填充柱的局限性，但吸附膜的制备需要严格的化学修饰。混合基质膜（MMM）的制备概念可以用作通过将吸附性树脂与膜聚合物溶液进行物理共混来制备膜色谱的替代方法。在当前的研究中，相对于基础聚合物含量，使用7.5 wt％的CNP105阳离子树脂和42.5 wt％的MP500阴离子树脂开发了多种相互作用的MMM色谱法，用于乳清蛋白分级分离。将树脂以不同的成分共混在EVAL和纤维素基础聚合物基质中。基于HPLC和SDS-PAGE分析，在一次乳清分馏中，酸性和碱性乳清蛋白均与多重相互作用MMM结合。使用基于EVAL的MMM对主要酸性乳清蛋白的结合能力为4.255 mg BSA结合/ g MMM，60.887 mgα-Lac结合/ g MMM和231.788 mgβ-Lac/ g MMM。对于基于CA的MMM，结合能力为2.970mg BSA结合/ g MMM，42.392mgα-Lac结合/ g MMM和179.817mgβ-Lac/ g MMM。

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    Shiew Chan Fong;

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  • 年度 2013
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