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Moderate halophilic bacteria colonizing the phylloplane of halophytes of the subfamily Salicornioideae (Amaranthaceae)

机译：中度嗜盐细菌定居在ali科亚科（A菜科）的盐生植物的叶平面上

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© 2015 Elsevier GmbH. Halophytes accumulate large amounts of salt in their tissues, and thus are susceptible to colonization by halotolerant and halophilic microorganisms that might be relevant for the growth and development of the plant. Here, the study of 814 cultured strains and 14,189 sequences obtained by 454 pyrosequencing were combined in order to evaluate the presence, abundance and diversity of halophilic, endophytic and epiphytic microorganisms in the phytosphere of leaves of members of the subfamily Salicornioideae from five locations in Spain and Chile. Cultures were screened by the tandem approach of MALDI-TOF/MS and 16S rRNA gene sequencing. In addition, differential centrifugation was used to enrich endophytes for further DNA isolation, 16S rRNA gene amplification and 454 pyrosequencing. Culturable and non-culturable data showed strong agreement with a predominance of Proteobacteria, Firmicutes and Actinobacteria. The most abundant isolates corresponded to close relatives of the species Chromohalobacter canadensis and Salinicola halophilus that comprised nearly 60% of all isolates and were present in all plants. Up to 66% of the diversity retrieved by pyrosequencing could be brought into pure cultures and the community structures were highly dependent on the compartment where the microorganisms thrived (plant surface or internal tissues).
机译：©2015 Elsevier GmbH。盐生植物在其组织中积累大量的盐，因此易受可能与植物的生长和发育有关的耐盐和嗜盐微生物定植。在这里，结合研究通过454焦磷酸测序获得的814个培养菌株和14,189个序列，以评估来自西班牙五个地方的S科亚科成员Salicornioideae叶片的植物层中嗜盐，内生和附生微生物的存在，丰度和多样性。和智利。通过MALDI-TOF / MS和16S rRNA基因测序的串联方法筛选培养物。另外，使用差速离心来富集内生菌，以用于进一步的DNA分离，16S rRNA基因扩增和454焦磷酸测序。可耕种和不可耕种的数据表明，主要与变形杆菌，硬毛菌和放线菌一致。最丰富的分离株对应于加拿大嗜热色杆菌和嗜盐梭菌（Salinicola halophilus）物种的近亲，它们占所有分离株的近60％，并存在于所有植物中。通过焦磷酸测序获得的多样性中，多达66％可以引入纯培养物中，并且群落结构高度依赖于微生物繁衍的隔室（植物表面或内部组织）。

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