• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >In vitro enfolding of olfactory neurites by p75 NGF receptor positive ensheathing cells from adult rat olfactory bulb
【2h】

In vitro enfolding of olfactory neurites by p75 NGF receptor positive ensheathing cells from adult rat olfactory bulb

机译：成年大鼠嗅球中p75 NGF受体阳性鞘细胞对嗅神经突的体外包裹

页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Secondary cultures of adult rat olfactory bulb (OB) contained three different types of cells (i) process-bearing cells; (ii) macrophage-like cells and (iii) fusiform cells. The immunohistochemical properties of process-bearing cells closely corresponded to those described for ensheathing glia in vivo. The most distinctive feature of these cells was their immunoreactivity for low affinity nerve growth factor receptor (NGFR). Process-bearing cells also shared the ultrastructural properties of ensheathing glia in vivo, as well as the ability to ensheath olfactory axons. In contrast, macrophage-like cells had the immunostaining properties of microglia, and fusiform cells were likely capillary endothelial cells. Neurites outgrowing from olfactory epithelium explants, when co-cultured with adult OB cells, grew preferentially over NGFR positive cells. Olfactory neurites exhibited NGFR immunoreactivity and were enfolded by NGFR positive cells. After ensheathment, this immunoreactivity decreased from the neurite and disappeared from the glial membrane in contact with the neurite. However, NGFR immunoreactivity was maintained in the portion of the glial membrane not involved in ensheathing. In summary, ensheathing cells in vitro retained both the ultrastructure shown in vivo and the ability to ensheath olfactory neurites. The Schwann cell-like properties of ensheathing glia, could partially explain the permissibility of adult OB to axonal growth.
机译：成年大鼠嗅球（OB）的二次培养物中包含三种不同类型的细胞：（i）带有过程的细胞； （ii）巨噬细胞样细胞和（iii）梭状细胞。带有过程的细胞的免疫组织化学性质与体内包被神经胶质细胞所描述的那些紧密对应。这些细胞最独特的特征是它们对低亲和力神经生长因子受体（NGFR）的免疫反应性。具有过程的细胞还具有在体内包裹神经胶质细胞的超微结构特性，以及包裹嗅觉轴突的能力。相反，巨噬细胞样细胞具有小胶质细胞的免疫染色特性，梭形细胞可能是毛细血管内皮细胞。与成年OB细胞共培养时，从嗅上皮外植体中长出的神经突优先于NGFR阳性细胞生长。嗅神经突表现出NGFR免疫反应性，并被NGFR阳性细胞包裹。包被后，该免疫反应性从神经突减少，并在与神经突接触的神经胶质膜上消失。但是，NGFR免疫反应性保持在胶膜的不参与鞘的部分。总而言之，体外包被细胞既保留了体内显示的超微结构，又保留了嗅觉神经突的能力。鞘胶质细胞的雪旺氏细胞样特性，可以部分解释成年OB对轴突生长的容许性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号