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首页> 外文OA文献 >Distribution of the serotonin 5-HT2 receptor family mRNAs: Comparison between 5-HT(2A) and 5-HT(2C) receptors
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Distribution of the serotonin 5-HT2 receptor family mRNAs: Comparison between 5-HT(2A) and 5-HT(2C) receptors

机译：血清素5-HT2受体家族mRNA的分布：5-HT（2A）和5-HT（2C）受体之间的比较

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Because of their similarities, serotonin 5-HT2, 5-HT(1C), and the recently described 5-HT(2F) receptors have been classified as members of the 5-HT2 receptor family, and they have been renamed 5-HT(2A), 5-HT(2C) and 5-HT(2B), respectively. The regional distribution and cellular localization of mRNA coding for the members of 5-HT2 receptor family were investigated in consecutive tissue sections from the rat brain by in situ hybridization histochemistry. No evidence for the expression of 5-HT(2B) receptor was found. High levels of 5-HT(2A) (formerly 5-HT2) receptor mRNA were observed only in few areas, as the frontal cortex, piriform cortex, ventro-caudal part of CA3, medial mammilary nucleus, the pontine nuclei and the motor cranial nerve nuclei in the brainstem, and the ventral horn of the spinal cord. The distribution of 5-HT(2A) receptor mRNA is generally in good agreement with that of the corresponding binding sites, although discrepancies were sometimes observed. 5-HT(2C) (formerly 5-HT(1c)) mRNA was present at very high levels in the choroid plexuses. However, very high levels were also seen in many other brain regions, as the retrosplenial, piriform and entorhinal cortex, anterior olfactory nucleus, lateral septal nucleus, subthalamic nucleus, amygdala, subiculum and ventral part of CA3, lateral habenula, substantia nigra pars compacta, several brainstem nuclei and the whole grey matter of the spinal cord. These results confirm and extend previous observations that 5-HT(2C) receptor mRNA is present in many brain areas in addition to those autoradiographically shown to have the corresponding binding sites and that 5-HT(2C) receptor subtype is a principal 5-HT receptor in the brain. From the comparison between their distributions, 5-HT(2A) and 5-HT(2C) receptor mRNAs appeared to be expressed in distinct but overlapping sets of brain regions. Both mRNAs coexisted at high levels in the anterior olfactory nucleus, piriform cortex, endopiriform nucleus, claustrum, pyramidal cell layer of the ventral part of CA3, taenia tecta, substantia nigra pars compacta, and several brainstem nuclei. In other regions both mRNAs were present but with different distributions, as the caudate-putamen. These results are also discussed in relation to the physiological meaning of the existence of two so similar receptor subtypes in the brain.
机译：由于它们的相似性，血清素5-HT2、5-HT（1C）和最近描述的5-HT（2F）受体已被归类为5-HT2受体家族的成员，它们被重命名为5-HT（ 2A），5-HT（2C）和5-HT（2B）。通过原位杂交组织化学研究了大鼠大脑连续组织切片中编码5-HT2受体家族成员的mRNA的区域分布和细胞定位。找不到5-HT（2B）受体表达的证据。仅在少数区域观察到高水平的5-HT（2A）（以前为5-HT2）受​​体mRNA，如额叶皮层，梨状皮层，CA3的腹尾部，乳腺内侧核，桥脑核和运动性颅脑脑干中的神经核和脊髓的腹角。 5-HT（2A）受体mRNA的分布通常与相应的结合位点相吻合，尽管有时会观察到差异。 5-HT（2C）（以前为5-HT（1c））mRNA在脉络丛中的含量很高。然而，在其他许多大脑区域，如脾后，皮状和内嗅皮层，前嗅核，外侧中隔核，丘脑下核，杏仁核，下丘脑和CA3腹侧部分，外侧ha，黑质致密部，也观察到很高的水平。 ，几个脑干核和整个脊髓灰质。这些结果证实并扩展了先前的观察结果，除了放射自显影显示具有相应结合位点的5-HT（2C）受体mRNA还存在于许多脑区域，并且5-HT（2C）受体亚型是主要的5-HT大脑中的受体。从它们的分布之间的比较，5-HT（2A）和5-HT（2C）受体mRNA似乎在不同但重叠的大脑区域集中表达。两种mRNA均以高水平共存于嗅觉前核，梨状皮层，内膜状核，锁骨锁骨，CA3腹侧的锥体细胞层，ta虫，黑质致密粉和几个脑干核。在其他区域，两个mRNA均存在，但分布不同，如尾状-丘脑。还就大脑中两种如此相似的受体亚型的存在的生理意义讨论了这些结果。

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