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首页> 外文OA文献 >Partial Substitution of Fishmeal by Meat and Bone Meal, Soybean Meal, and Squid Concentrate in Feeds for the Prawn, Artemesia longinaris: Effect on Digestive Proteinases
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Partial Substitution of Fishmeal by Meat and Bone Meal, Soybean Meal, and Squid Concentrate in Feeds for the Prawn, Artemesia longinaris: Effect on Digestive Proteinases

机译：鱼粉被肉和骨粉，大豆粉和鱿鱼浓缩液替代，用于虾，长蒿的饲料：对消化蛋白酶的影响

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摘要

The effect of alternative protein sources on proteinase activity in the midgut gland of Artemesia longinaris was studied. Three isoproteic feeds were compared: a basal diet containing 48% fishmeal and 17% soybean meal (diet 1), the basal diet containing meat and bone meal as partial replace- ment of the fishmeal (diet 2), the basal diet containing additional soybean meal and a squid protein concentrate as partial replacement of the fishmeal (diet 3). Midgut gland extracts from the three treatments and wild prawns (control) were assayed to quantify proteinase activity. Specific inhibitors were used to identify proteinase classes by SDS-PAGE. Specific proteolytic activity was highest in the wild prawns and in prawns fed diet 2. Trypsin ac-tivity was higher in prawns fed the diets (1.9-2.6 absorbance/min/mg) than in wild prawns (0.6 absorbance/min/mg). Chymotrypsin activity was high- est in prawns fed diet 2. Proteinase activity in samples on azocasein was inhibited by soybean trypsin inhibitor and Nα-p-tosyl-L-lysine chloromethyl ketone. Electrophoresis revealed at least six different bands with zones having caseinolytic activity in prawns fed the diets as compared to wild prawns, including four trypsins (16.6, 18.2, 21.9, and 25.1 kDa) and one chymotrypsin (53.7 kDa). Our findings indicate that proteolytic activity in A. longinaris adapts to the quality of the dietary protein and that fishmeal can partially be replaced by additional soybean meal in combination with squid protein concentrate.
机译：研究了替代蛋白质来源对长蒿蒿中肠腺蛋白酶活性的影响。对三种同蛋白饲料进行了比较：一种基本饮食包含48％的鱼粉和17％的豆粕（饮食1），该基本饮食包含肉和骨粉作为鱼粉的部分替代品（饮食2），该基本饮食包含额外的大豆粗粉和鱿鱼蛋白浓缩物作为鱼粉的部分替代品（饮食3）。分析了来自三种处理和野生虾（对照）的中肠腺提取物，以定量蛋白酶活性。使用特异性抑制剂通过SDS-PAGE鉴定蛋白酶类别。在野生虾和饲喂日粮的虾中，蛋白水解活性最高。饲喂日粮的虾（1.9-2.6吸光度/ min / mg）的胰蛋白酶活性高于野生虾（0.6吸光度/ min / mg）。饲喂日粮2的虾中胰凝乳蛋白酶的活性最高。大豆胰蛋白酶抑制剂和Nα-对-甲苯基-L-赖氨酸氯甲基酮抑制了偶氮酪蛋白样品的蛋白酶活性。电泳显示，与野生虾相比，饲喂日粮的虾至少有六个不同的带，具有酪蛋白分解活性区域，其中包括四个胰蛋白酶（16.6、18.2、21.9和25.1 kDa）和一个胰凝乳蛋白酶（53.7 kDa）。我们的发现表明，A。longinaris中的蛋白水解活性与膳食蛋白质的质量相适应，鱼粉可以部分地由豆粕和鱿鱼浓缩蛋白的组合来代替。

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