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Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases

机译：Dun1和Rad53蛋白激酶FHA结构域的结晶和初步X射线衍射研究

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摘要

Forkhead-associated (FHA) domains are modular protein–protein interaction domains of ~130 amino acids present in numerous signalling proteins. FHA-domain-dependent protein interactions are regulated by phosphorylation of target proteins and FHA domains may be multifunctional phosphopeptide-recognition modules. FHA domains of the budding yeast cell-cycle checkpoint protein kinases Dun1p and Rad53p have been crystallized. Crystals of the Dun1-FHA domain exhibit the symmetry of the space group P6122 or P6522, with unit-cell parameters a = b = 127.3, c = 386.3 Å; diffraction data have been collected to 3.1 Å resolution on a synchrotron source. Crystals of the N-terminal FHA domain (FHA1) of Rad53p diffract to 4.0 Å resolution on a laboratory X-ray source and have Laue-group symmetry 4/mmm, with unit-cell parameters a = b = 61.7, c = 104.3 Å.

机译：前叉相关（FHA）域是许多信号蛋白中存在的约130个氨基酸的模块化蛋白质-蛋白质相互作用域。 FHA结构域依赖性蛋白相互作用通过靶蛋白的磷酸化来调节，而FHA结构域可能是多功能的磷酸肽识别模块。发芽的酵母细胞周期检查点蛋白激酶Dun1p和Rad53p的FHA结构域已经结晶。 Dun1-FHA结构域的晶体表现出空间群P6122或P6522的对称性，其晶胞参数a = b = 127.3，c = 386.3Å；已在同步加速器源上以3.1Å的分辨率收集了衍射数据。 Rad53p的N端FHA结构域（FHA1）晶体在实验室X射线源上衍射至4.0Å分辨率，Laue基对称性为4 / mmm，晶胞参数a = b = 61.7，c = 104.3Å 。

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Blanchard H.; Fontes R. M; Hammet A.; Pike B. L.; Teh T.; Gleichmann T.; Gooley P. R.; Kobe B.; Heierhorst J.;
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年度 2001
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1. Direct Kinase-to-Kinase Signaling Mediated by the FHA Phosphoprotein Recognition Domain of the Dun1 DNA Damage Checkpoint Kinase [J] . Vladimir I. Bashkirov, Elena V. Bashkirova, Edwin Haghnazari, Molecular and Cellular Biology . 2003,第4期

机译：Dun1 DNA损伤检查点激酶的FHA磷酸蛋白识别域介导的直接激酶转激酶信号传导。
2. Crystallization and preliminary X-ray diffraction analyses of the TIR domains of three TIR-NB-LRR proteins that are involved in disease resistance in Arabidopsis thaliana [J] . Wan Li, Zhang Xiaoxiao, Williams Simon J., Acta crystallographica, Section F. Structural biology and crystallization communications . 2013,第11期

机译：三种拟南芥抗病性相关的TIR-NB-LRR蛋白的TIR结构域的结晶和初步X射线衍射分析
3. Purification, isolation, crystallization, and preliminary X-ray diffraction study of the BTB domain of the centrosomal protein 190 from Drosophila melanogaster [J] . Boyko K. M., Nikolaeva A. Yu., Kachalova G. S., Crystallography reports . 2017,第6期

机译：从德罗洛拉美洛拉斯替斯科斯州的Centomal蛋白190的BTB结构域的纯化，分离，结晶和初步X射线衍射研究
4. Identification of compensatory phosphorylation and interactors of checkpoint kinase Rad53 responding to defective Dun1 activation in normal S phase [C] . Eric S.-W. Chen, Ming-Daw Tsai American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：检查点激酶Rad53响应正常S期缺陷DUN1活化的补偿性磷酸化和交互因子
5. Stringent starvation protein A: Expression, purification, characterization, and preliminary x-ray diffraction studies [D] . Andrykovitch, Michelle 2002

机译：严格的饥饿蛋白A：表达，纯化，表征和初步X射线衍射研究
6. Direct Kinase-to-Kinase Signaling Mediated by the FHA Phosphoprotein Recognition Domain of the Dun1 DNA Damage Checkpoint Kinase [O] . Vladimir I. Bashkirov, Elena V. Bashkirova, Edwin Haghnazari, 2003

机译：Dun1 DNA损伤检查点激酶的FHA磷酸蛋白识别域介导的直接激酶转激酶信号传导。
7. Crystallisation and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases. [O] . Blanchard, Helen, Fontes, M.M, Pike, B.L., 2001

机译：Dun1和Rad53蛋白激酶FHA结构域的结晶和初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of FHA domains of Dun1 and Rad53 protein kinases

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