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Molecular Methods for Identification of Acinetobacter Species by Partial Sequencing of the rpoB and 16S rRNA Genes.

机译：通过对rpoB和16S rRNA基因进行部分测序来鉴定不动杆菌种类的分子方法。

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BACKGROUNDududAcinetobacter spp. is a diverse group of Gram-negative bacteria which are ubiquitous in soil and water, and an important cause of nosocomial infections. The purpose of this study was to identify a collection of Acinetobacter spp. clinical isolates accurately and to investigate their antibiotic susceptibility patterns.ududMATERIALS AND METHODSududA total of 197 non-duplicate clinical isolates of Acinetobacter spp. isolates identified using conventional biochemical tests. The molecular technique of PCR-RFLP and sequence analysis of rpoB and 16S rRNA genes was applied for species identification. Antimicrobial susceptibility test was performed with a disk diffusion assay.ududRESULTSududBased on 16S rRNA and rpoB genes analysis separately, most of clinical isolates can be identified with high bootstrap values. However, the identity of the isolate 555T was uncertain due to high similarity of A. grimontii and A. junii. Identification by concatenation of 16S rRNA and rpoB confirmed the identity of clinical isolates of Acenitobacer to species level confidently. Accordingly, the isolate 555T assigned as A. grimontii due to 100% similarity to A. grimontii. Moreover, this isolate showed 98.64% to A. junii. Besides, the identity of the isolates 218T and 364T was confirmed as Genomic species 3 and A. calcoaceticus respectively. So, the majority of Acinetobacter spp. isolates, were identified as: A. baumannii (131 isolates, 66%), A. calcoaceticus (9 isolates, 4.5%), and A. genomosp 16 (8 isolates, 4%). The rest of identified species showed the lower frequencies. In susceptibility test, 105 isolates (53%), presented high antibiotic resistance of 90% to ceftriaxone, piperacillin, piperacillin tazobactam, amikacin, and 81% to ciprofloxacin.ududCONCLUSIONududSequence analysis of the 16S rRNA and rpoB spacer simultaneously was able to do identification of Acinetobacter spp. to species level. A.baumannii was identified as the most prevalent species with high antibiotic resistance. Other species showed lower frequencies ranged from 4 to 9 strains.
机译：背景 ud ud不动杆菌属。革兰氏阴性菌是在土壤和水中普遍存在的多种革兰氏阴性细菌，是医院感染的重要原因。这项研究的目的是确定不动杆菌属的集合。 ud ud材料和方法 ud ud共有197种不重复的不动杆菌属细菌临床分离株。使用常规生化测试鉴定出的分离物。 PCR-RFLP的分子技术以及rpoB和16S rRNA基因的序列分析被用于物种鉴定。 ud udRESULTS ud ud分别基于16S rRNA和rpoB基因分析，可以鉴定出大多数具有高自举值的临床分离株。但是，由于grimontii和junii的高度相似性，因此分离物555T的身份尚不确定。通过串联16S rRNA和rpoB进行的鉴定证实了Acenitobacer的临床分离株在物种水平上的可信度。因此，分离株555T由于与grimontii有100％的相似性而被指定为grimontii。此外，该分离物显示出对A. junii的98.64％。此外，分离株218T和364T的身份分别被确认为基因组种3和钙乙酸曲霉。因此，大多数不动杆菌属。分离物被鉴定为：鲍曼不动杆菌（131个分离物，占66％），钙乙酸曲霉（9个分离物，占4.5％）和Genomosp 16（8个分离物，占4％）。其余已鉴定物种显示出较低的频率。在药敏试验中，有105个分离株（53％）对头孢曲松，哌拉西林，哌拉西林他唑巴坦，丁胺卡那霉素和环丙沙星的耐药率高达90％。 ud ud结论 ud ud16S rRNA和rpoB间隔子的序列分析同时能够鉴定不动杆菌属。到物种级别。鲍曼不动杆菌被鉴定为具有高抗生素抗性的最普遍的物种。其他物种显示的低频范围为4至9个菌株。

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