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首页> 外文OA文献 >The sucrose transporter HbSUT3 plays an active role in sucrose loading to laticifer and rubber productivity in exploited trees of Hevea brasiliensis (para rubber tree).
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The sucrose transporter HbSUT3 plays an active role in sucrose loading to laticifer and rubber productivity in exploited trees of Hevea brasiliensis (para rubber tree).

机译：蔗糖转运蛋白HbSUT3在巴西橡胶树（橡胶树）的砍伐树木中，对蔗糖的装载量起到胶基化作用，促进橡胶生产力。

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摘要

Efficient sucrose loading in rubber-producing cells (laticifer cells) is essential for retaining rubber productivity in Hevea brasiliensis, but the molecular mechanisms underlying the regulation of this process remain unknown. Here, we functionally characterized a putative Hevea SUT member, HbSUT3, mainly in samples from regularly exploited trees. When expressed in yeast, HbSUT3 encodes a functional sucrose transporter that exhibits high sucrose affinity with a K(m) value of 1.24 mm at pH 4.0, and possesses features typical of sucrose/H(+) symporters. In planta, when compared to the expression of other Hevea SUT genes, HbSUT3 was found to be the predominant member expressed in the rubber-containing cytoplasm (latex) of laticifers. The comparison of HbSUT3 expression among twelve Hevea tissues demonstrates a relatively tissue-specific pattern, i.e. expression primarily in the latex and in female flowers. HbSUT3 expression is induced by the latex stimulator Ethrel (an ethylene generator), and relates to its yield-stimulating effect. Tapping (the act of rubber harvesting) markedly increased the expression of HbSUT3, whereas wounding alone had little effect. Moreover, the expression of HbSUT3 was found to be positively correlated with latex yield. Taken together, our results provide evidence favouring the involvement of HbSUT3 in sucrose loading into laticifers and in rubber productivity.
机译：橡胶生产细胞（胶乳细胞）中有效的蔗糖负载量对于维持巴西橡胶树橡胶的生产力至关重要，但是调控该过程的分子机制仍然未知。在这里，我们在功能上对推定的三叶胶SUT成员HbSUT3进行了功能表征，主要是从定期采伐的树木中取样。当在酵母中表达时，HbSUT3编码一种功能性蔗糖转运蛋白，该蔗糖转运蛋白在pH 4.0时显示出高蔗糖亲和力，K（m）值为1.24 mm，并具有蔗糖/ H（+）转运蛋白的典型特征。在植物中，与其他Hevea SUT基因的表达相比，HbSUT3是在胶乳的含橡胶细胞质（胶乳）中表达的主要成员。 HbSUT3在十二种橡胶树组织中表达的比较表明，组织具有相对特定的模式，即主要在乳胶和雌花中表达。 HbSUT3的表达是由乳胶刺激剂Ethrel（一种乙烯产生剂）诱导的，并且与其刺激产量有关。敲击（收获橡胶的行为）显着增加了HbSUT3的表达，而单独受伤几乎没有影响。此外，发现HbSUT3的表达与乳胶产量正相关。两者合计，我们的结果提供了证据，证明HbSUT3参与将蔗糖装载到胶凝剂中和橡胶生产率方面。

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