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Convection-compensating PGSE experiment incorporating excitation-sculpting water suppression (CONVEX)

机译:结合激发雕刻水抑制(CONVEX)的对流补偿PGSE实验

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摘要

We present a new diffusion experiment which provides simultaneous suppression of an on-resonance solvent peak and compensation for convection. The experiment, which we name CONVEX, exploits similarities between two functionally different pulse sequences to enable the same sequence to be used simultaneously for two different purposes. The CONVEX pulse sequence combines a double-echo PGSE with double excitation-sculpting water suppression, using unequal gradient pulse-pair amplitudes (g1 and g2) and unequal diffusion intervals (Del1 and Del2). Convection compensation is achieved by setting g1 : g2 = Del2 : Del1. The new experiment provides the spectral quality, flat baseline, and water-suppression power characteristic of excitation-sculpting experiments, combined with excellent compensation for convection. The resulting Stejskal–Tanner plots are linear over a greater range of signal attenuation than in the absence of water suppression. Possible applications include protein NMR; NMR of cellular or colloidal systems; and the monitoring of technological processes.
机译:我们提出了一个新的扩散实验,该实验可以同时抑制共振溶剂峰和对流补偿。我们将其命名为CONVEX的实验利用了两个功能不同的脉冲序列之间的相似性,以使同一序列可以同时用于两个不同的目的。 CONVEX脉冲序列使用不相等的梯度脉冲对幅度(g1和g2)和不相等的扩散间隔(Del1和Del2)将双回波PGSE与双重激励雕刻水抑制相结合。对流补偿通过设置g1:g2 = Del2:Del1来实现。新的实验提供了激发雕刻实验的光谱质量,平坦的基线和抑水能力特征,并具有出色的对流补偿能力。与没有水抑制的情况相比,在更大的信号衰减范围内,所得的Stejskal–Tanner图是线性的。可能的应用包括蛋白质NMR;细胞或胶体系统的NMR;以及对工艺流程的监控。

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