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Characterization of goblet cells in a pterygium biopsy using laser scanning confocal microscopy and immunohistochemistry

机译：翼状ery肉活检中杯状细胞的特征使用激光扫描共聚焦显微镜和免疫组织化学

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udud - To confirm that structures presumed to be GCs observed using are actually GCs.udududud - A single tissue sample was obtained from a pterygium that was freshly excised from a 33-year-old male. After viewing what were believed to be GCs in the tissue sample using LSCM, the same sample was observed using laboratory confocal microscopy and immunohistochemistry. GCs were identified by a combination of classic morphologic appearance and the use of immunofluorescence to antibodies for mucin 5AC and cytokeratin-7. The LSCM and immunohistochemistry results were compared.udududud - Using LSCM, GCs were observed between 7 and 41 μm deep, at the level of the superficial basal cells of the tissue sample. GCs were estimated to have a diameter of 35-40 μm near the surface and 20-30 μm in the deeper layers. A small dark dot was visible in some GCs, indicating cell nuclei and/or the opened apical portion of cells representing the site of mucin release. GCs were more reflective and larger than the surrounding cells. Positively stained GCs in immunofluorescence showed a similar distribution pattern to those observed with LSCM. The tissue sample stained intensely for GC-specific mucin type 5AC.udududud - The pattern of discrete, large reflective cells observed using LSCM are likely to be GCs.
机译：ud ud-要确认推测使用GC观察到的结构实际上是GC。 ud ud ud ud-从翼状g肉中获得的单个组织样本是从一个33岁的男性身上新鲜切除的。使用LSCM查看组织样本中被认为是GC的物质后，使用实验室共聚焦显微镜和免疫组织化学观察到了同一样本。通过结合经典形态学外观和使用针对粘蛋白5AC和细胞角蛋白7的抗体的免疫荧光技术来鉴定GC。比较LSCM和免疫组织化学结果。 ud ud ud ud-使用LSCM，在组织样品的浅层基底细胞水平上观察到7至41μm深的GC。据估计，GC的直径在表面附近为35-40μm，在较深层为20-30μm。在某些GC中可见一个小的黑点，表明细胞核和/或代表粘蛋白释放位点的开放的细胞顶端部分。 GC比周围的单元格更具反射性且更大。免疫荧光中阳性染色的GC与LSCM观察到的分布模式相似。组织样本对GC特异性粘蛋白5AC型进行了强烈染色。 ud ud ud ud-使用LSCM观察到的离散的大型反射细胞的模式很可能是GC。

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