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首页> 外文OA文献 >Discovery, structural characterization and functional analysis of alpha-2-macroglobulin, a novel immune-related molecule from Holothuria atra
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Discovery, structural characterization and functional analysis of alpha-2-macroglobulin, a novel immune-related molecule from Holothuria atra

机译：来自海参的新型免疫相关分子α-2-巨球蛋白的发现，结构表征和功能分析

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The non-specific protease inhibitor alpha-2-macroglobulin (A(2)M) is a key macromolecular glycoprotein that involved in host immune defense against pathogens in vertebrates and invertebrates. However, no research regarding A(2)M has been developed in echinoderms to date. In this study, the full-length cDNA of A(2)M was cloned from-the sea cucumber (Holothuria atra), which is a tropical species widely distributed along the coasts of the South China Sea and designated HaA(2)M. HaA(2)M possesses all three conserved functional domains of known A(2)M proteins, including the bait region domain, thioester domain and receptor-binding domain. Compared to fish and shrimp A(2)Ms, the histidine residue from the catalytical regions is well conserved in HaA(2)M. HaA(2)M mRNA was predominantly expressed in coelomocytes and, to a lesser extent, in the body wall, intestine and respiratory tree. A(2)M activity was detected in the coelomic fluids of H. atra. The mRNA expression and activity levels were investigated in the major immune tissues and coelomic fluids of H. atra after challenge with inactivated Vibrio alginolyticus or polyriboinosinic polyribocytidylic acid [Poly (I: C)]. RNA interference (RNAi)-mediated knockdown of HaA(2)M resulted in a significant reduction of HaA(2)M gene transcript level (86%). RNAi-mediated silencing of HaA(2)M gene significantly decreased the A(2)M activity (38%) and increased the number of viable bacteria (2.8-fold) in the coelomic fluids of H. atra infected by V. alginolyticus. Our study, as a whole, supplied the evidences for HaA(2)M as an immune-relevant molecule and it might have multiple functions in the innate immune system of H. atra. (C) 2016 Elsevier B.V. All rights reserved.
机译：非特异性蛋白酶抑制剂α-2-巨球蛋白（A（2）M）是关键的大分子糖蛋白，参与宿主对脊椎动物和无脊椎动物中病原体的免疫防御。但是，迄今尚未在棘皮动物中进行有关A（2）M的研究。在这项研究中，从海参（Holothuria atra）克隆了A（2）M的全长cDNA，海参是在南中国海沿岸广泛分布的热带物种，命名为HaA（2）M。 HaA（2）M拥有已知A（2）M蛋白的所有三个保守功能结构域，包括诱饵区结构域，硫酯结构域和受体结合结构域。与鱼类和虾A（2）Ms相比，来自催化区域的组氨酸残基在HaA（2）M中保存良好。 HaA（2）M mRNA主要在体细胞中表达，并在较小程度上在体壁，肠和呼吸道树中表达。 A.（2）M活性被检测到的tra.Htra腔腔积液。用灭活的溶藻弧菌或聚核糖核酸聚核糖核酸[Poly（I：C）]攻击后，研究了H. atra的主要免疫组织和腔液中的mRNA表达和活性水平。 HaA（2）M的RNA干扰（RNAi）介导的敲低导致HaA（2）M基因转录水平显着降低（86％）。 Hai（2）M基因的RNAi介导的沉默大大降低了A（2）M的活性（38％），并增加了溶藻弧菌感染的H. atra体液中活细菌的数量（2.8倍）。总体而言，我们的研究提供了HaA（2）M作为一种与免疫相关的分子的证据，并且它在H.atra的固有免疫系统中可能具有多种功能。 （C）2016 Elsevier B.V.保留所有权利。

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